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Literature summary extracted from

  • Nishikawa, Y.; Pegg, W.; Paulsen, H.; Schachter, H.
    Control of glycoprotein synthesis. Purification and characterization of rabbit liver UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (1988), J. Biol. Chem., 263, 8270-8281.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.101 additional information no activation by 2-mercaptoethanol Oryctolagus cuniculus
2.4.1.101 Triton X-100
-
Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.101 2'-dUDP weak Oryctolagus cuniculus
2.4.1.101 2'-O-Methyl-UDP weak Oryctolagus cuniculus
2.4.1.101 5-Bromo-UTP
-
Oryctolagus cuniculus
2.4.1.101 5-Mercury-UDP
-
Oryctolagus cuniculus
2.4.1.101 alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
Oryctolagus cuniculus
2.4.1.101 additional information no inhibitors are CDP, UDPhexanolamine, AMP Oryctolagus cuniculus
2.4.1.101 TDP
-
Oryctolagus cuniculus
2.4.1.101 UDP
-
Oryctolagus cuniculus
2.4.1.101 UDP-glucose weak Oryctolagus cuniculus
2.4.1.101 UDP-N-acetylgalactosamine weak Oryctolagus cuniculus
2.4.1.101 UDP-N-acetylglucosamine competitively to UDP Oryctolagus cuniculus
2.4.1.101 UMP weak Oryctolagus cuniculus
2.4.1.101 UTP weak Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.101 additional information
-
additional information kinetic study Oryctolagus cuniculus
2.4.1.101 0.0384
-
UDP-N-acetyl-D-glucosamine
-
Oryctolagus cuniculus
2.4.1.101 0.0384
-
alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
Oryctolagus cuniculus
2.4.1.101 2.03
-
alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.101 membrane
-
Oryctolagus cuniculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.101 Ba2+ slight activation Oryctolagus cuniculus
2.4.1.101 Ca2+ slight activation Oryctolagus cuniculus
2.4.1.101 Cd2+ activation Oryctolagus cuniculus
2.4.1.101 Cd2+ can substitute for Mn2+ with 20% Oryctolagus cuniculus
2.4.1.101 Co2+ activation Oryctolagus cuniculus
2.4.1.101 Co2+ can substitute for Mn2+ with 80% Oryctolagus cuniculus
2.4.1.101 Fe2+ activation Oryctolagus cuniculus
2.4.1.101 Fe2+ can substitute for Mn2+ with 65% efficiency Oryctolagus cuniculus
2.4.1.101 Mg2+ activation Oryctolagus cuniculus
2.4.1.101 Mg2+ can substitute for Mn2+ with 40% Oryctolagus cuniculus
2.4.1.101 Mn2+ requirement Oryctolagus cuniculus
2.4.1.101 additional information no activation by Cu2+ Oryctolagus cuniculus
2.4.1.101 additional information no activation by Sr2+, Pb2+ Oryctolagus cuniculus
2.4.1.101 Ni2+ activation Oryctolagus cuniculus
2.4.1.101 Ni2+ can substitute for Mn2+ with 20% Oryctolagus cuniculus
2.4.1.101 Sn2+ slight activation Oryctolagus cuniculus
2.4.1.101 Zn2+ activation Oryctolagus cuniculus
2.4.1.101 Zn2+ can substitute for Mn2+ with 8% Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.101 additional information
-
separable by gel filtration Oryctolagus cuniculus
2.4.1.101 additional information
-
two MW-species Oryctolagus cuniculus
2.4.1.101 45000
-
x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 Oryctolagus cuniculus
2.4.1.101 50000
-
x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 Oryctolagus cuniculus
2.4.1.101 54000
-
x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide Oryctolagus cuniculus key enzyme of biosynthesis of complex and hybrid N-glycans UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.101 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.101 two molecular weight forms separable by gel filtration, purification of low-molecular weight form Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.101 UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] mechanism Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.101 liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.101 19.8
-
low-molecular weight form Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.101 additional information acts on synthetic substrate analogues Oryctolagus cuniculus ?
-
?
2.4.1.101 additional information no acceptor substrates are N-acetyl-D-glucosamine, N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn Oryctolagus cuniculus ?
-
?
2.4.1.101 UDP + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
Oryctolagus cuniculus ?
-
?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R 1,4-N-acetyl-D-glucosaminyl-Asn Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R synthetic-beta-D-mannosyl-(1,6-anhydro)-derivative acts as substrate, too Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide key enzyme of biosynthesis of complex and hybrid N-glycans Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide
-
?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R substrate specificity Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: 1,4-N-acetyl-D-glucosaminyl-Asn ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R essential for activity: unsubstituted equatorial hydroxyl on C-4 of beta-linked mannosyl-residue Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: 1,4-N-acetyl-D-glucosaminyl-Asn ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: 1,4-N-acetyl-D-glucosaminyl-Asn Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: 1,4-N-acetyl-D-glucosaminyl-Asn ?

Subunits

EC Number Subunits Comment Organism
2.4.1.101 ? x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.101 37
-
assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.101 5.6
-
-
Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.101 5.1 6.9 about half-maximal activity at pH 5.1 and about 70% of maximal activity at pH 6.9 Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.101 0.034
-
UDP-N-acetylglucosamine
-
Oryctolagus cuniculus
2.4.1.101 1.8
-
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
Oryctolagus cuniculus