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Literature summary extracted from

  • Van den Ende, W.; Van Wonterghem, D.; Verhaert, P.; Dewil, E.; Van Laere, A.
    Purification and characterization of fructan: fructan fructosyl transferase from chicory roots (1996), Planta, 199, 493-502.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.100 Ag+
-
 Cichorium intybus
2.4.1.100 Cu2+
-
 Cichorium intybus
2.4.1.100 Hg2+
-
 Cichorium intybus
2.4.1.100 sucrose competitive inhibitor of donor substrates at 8-50 mM Cichorium intybus
2.4.1.100 Zn2+
-
 Cichorium intybus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.100 119
-
 1-kestose
-
 Cichorium intybus
2.4.1.100 152
-
 1,1-nystose
-
 Cichorium intybus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.100 17000
-
 1 * 17000 + 1 * 52000, SDS-PAGE Cichorium intybus
2.4.1.100 52000
-
 1 * 17000 + 1 * 52000, SDS-PAGE Cichorium intybus
2.4.1.100 69000
-
 gel filtration Cichorium intybus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.100 Cichorium intybus
-
 L. var. foliosum cv. Flash
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.100 glycoprotein
-
 Cichorium intybus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.100 root
-
 Cichorium intybus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.100 14.5
-
-
 Cichorium intybus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.100 1,1-nystose + 1,1-nystose
-
 Cichorium intybus sucrose + 1-kestose + oligofructan DP5 + oligofructan DP6
-
 ?
2.4.1.100 1-kestose + 1-kestose
-
 Cichorium intybus 1,1-nystose + 1,1,1-logose 1,1-nystose + sucrose + oligofructans DP5 and DP6 ?
2.4.1.100 sucrose + 1,1-nystose
-
 Cichorium intybus ?
-
 ?
2.4.1.100 sucrose + 1-kestose
-
 Cichorium intybus 1,1-nystose
-
 ?
2.4.1.100 sucrose + inulin
-
 Cichorium intybus oligosaccharide
-
 ?
2.4.1.100 sucrose + oligofructan
-
 Cichorium intybus ?
-
 ?
2.4.1.100 [beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
-
 Cichorium intybus [beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.100 dimer 1 * 17000 + 1 * 52000, SDS-PAGE Cichorium intybus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.100 5.5 6.5
-
 Cichorium intybus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.100 15
-
 sucrose
-
 Cichorium intybus