BRENDA - Enzyme Database

Purification and characterization of wheat beta(2->1) fructan:fructan fructosyl transferase activity

Jeong, B.R.; Housley, T.L.; Plant Physiol. 100, 199-204 (1992)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.1.100
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
Triticum aestivum
role of enzyme in synthesis of fructan
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.4.1.100
Triticum aestivum
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.4.1.100
-
Triticum aestivum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.100
leaf blade
-
Triticum aestivum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.4.1.100
1-kestose + 1-kestose
-
488349
Triticum aestivum
1,1-nystose + 1,1,1-logose
inulin-type tetra- and pentasaccharides
488349
Triticum aestivum
?
2.4.1.100
1-kestose + 6-kestose
-
488349
Triticum aestivum
1,6-nystose + 6,1-nystose
-
488349
Triticum aestivum
?
2.4.1.100
sucrose + 1-kestose
-
488349
Triticum aestivum
1,1-nystose
-
488349
Triticum aestivum
?
2.4.1.100
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
enzyme is specific for fructosyl transfer from beta-2,1-linked 1-kestose or fructan to sucrose and beta-2,1-fructosyl transfer to other fructans
488349
Triticum aestivum
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
-
488349
Triticum aestivum
?
2.4.1.100
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
role of enzyme in synthesis of fructan
488349
Triticum aestivum
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.100
30
-
-
Triticum aestivum
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.4.1.100
5
40
5°C: about 70% of maximal activity, 40°C: about 75% of maximal activity
Triticum aestivum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.100
6.5
-
-
Triticum aestivum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.100
5
8
pH 5.0: about 70% of maximal activity, pH 8.0: about 65% of maximal activity
Triticum aestivum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.1.100
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
Triticum aestivum
role of enzyme in synthesis of fructan
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.100
-
Triticum aestivum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.100
leaf blade
-
Triticum aestivum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.4.1.100
1-kestose + 1-kestose
-
488349
Triticum aestivum
1,1-nystose + 1,1,1-logose
inulin-type tetra- and pentasaccharides
488349
Triticum aestivum
?
2.4.1.100
1-kestose + 6-kestose
-
488349
Triticum aestivum
1,6-nystose + 6,1-nystose
-
488349
Triticum aestivum
?
2.4.1.100
sucrose + 1-kestose
-
488349
Triticum aestivum
1,1-nystose
-
488349
Triticum aestivum
?
2.4.1.100
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
enzyme is specific for fructosyl transfer from beta-2,1-linked 1-kestose or fructan to sucrose and beta-2,1-fructosyl transfer to other fructans
488349
Triticum aestivum
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
-
488349
Triticum aestivum
?
2.4.1.100
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
role of enzyme in synthesis of fructan
488349
Triticum aestivum
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.100
30
-
-
Triticum aestivum
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.4.1.100
5
40
5°C: about 70% of maximal activity, 40°C: about 75% of maximal activity
Triticum aestivum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.100
6.5
-
-
Triticum aestivum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.100
5
8
pH 5.0: about 70% of maximal activity, pH 8.0: about 65% of maximal activity
Triticum aestivum