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Literature summary extracted from

  • Hochstrasser, U.; Luscher, M.; De Virgilio, C.; Boller, T.; Wiemken, A.
    Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris (1998), FEBS Lett., 440, 356-360.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 expression in Escherichia coli Hordeum vulgare
2.4.1.10 expression in Pichia pastoris X-33 from plasmid containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor for entry into the secretory pathway, secretion into the medium Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.10 additional information enzyme functionally expressed in Pichia pastoris and secreted into the medium shows additional sucrose-sucrose 1-fructosyltransferase activity, methanol induction Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n Hordeum vulgare
-
 glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Hordeum vulgare
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.10 additional information
-
 levansucrase and additional beta-fructosidase, i.e. invertase, sucrose-sucrose 1-fructosyltransferase, and sucrose-sucrose 6-fructosyltransferase activities of recombinant enzyme from Pichia pastoris, overview Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n additional beta-fructosidase, i.e. invertase, sucrose-sucrose 1-fructosyltransferase, and sucrose-sucrose 6-fructosyltransferase activities of recombinant enzyme from Pichia pastoris Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 6-SFT
-
 Hordeum vulgare
2.4.1.10 sucrose:fructan 6-fructosyltransferase
-
 Hordeum vulgare