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Literature summary extracted from

  • Kim, H.; Park, H.E.; Kim, M.J.; Lee, H.G.; Yang, J.Y.; Cha, J.
    Enzymatic characterization of a recombinant levansucrase from Rahnella aquatilis ATCC 15552 (2003), J. Microbiol. Biotechnol., 13, 230-235.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 overexpression of His-tagged enzyme in Escherichia coli, gene lscR Rahnella aquatilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.10 intracellular recombinant enzyme expressed in Escherichia coli Rahnella aquatilis 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 49000
-
 x * 49000, recombinant enzyme, SDS-PAGE Rahnella aquatilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Rahnella aquatilis
-
 strain ATCC15552, lscR gene
-
2.4.1.10 Rahnella aquatilis ATCC 15552
-
 strain ATCC15552, lscR gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 recombinant overexpression in E. coli, His-tagged protein Rahnella aquatilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Rahnella aquatilis glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Rahnella aquatilis ATCC 15552 glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 ? x * 49000, recombinant enzyme, SDS-PAGE Rahnella aquatilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 30
-
 recombinant enzyme Rahnella aquatilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 6
-
 recombinant enzyme Rahnella aquatilis