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Literature summary extracted from

  • Ohtsuka, K.; Hino, S.; Fukushima, T.; Ozawa, O.; Kanematsu, T.; Uchida, T.
    Characterization of levansucrase from Rahnella aquatilis JCM-1683 (1992), Biosci. Biotechnol. Biochem., 56, 1373-1377.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.10 Iodine slight inhibition Rahnella aquatilis
2.4.1.10 additional information hydroxylamine hydrochloride; not affected by 2-mercaptoethanol; not: EDTA Rahnella aquatilis
2.4.1.10 Ni2+ slight inhibition Rahnella aquatilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.10 50
-
 sucrose
-
 Rahnella aquatilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 64000
-
 2 * 64000, SDS-PAGE Rahnella aquatilis
2.4.1.10 120000
-
 gel filtration Rahnella aquatilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Rahnella aquatilis
-
 strain JCM-1683
-
2.4.1.10 Rahnella aquatilis JCM -1683
-
 strain JCM-1683
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10
-
 Rahnella aquatilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.10 731
-
 purified enzyme Rahnella aquatilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n various D-fructosyl acceptors, overview Rahnella aquatilis glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n various D-fructosyl acceptors, overview Rahnella aquatilis JCM -1683 glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + cellobiose
-
 Rahnella aquatilis ?
-
 ?
2.4.1.10 sucrose + D-arabinose
-
 Rahnella aquatilis ?
-
 ?
2.4.1.10 sucrose + D-maltose
-
 Rahnella aquatilis ?
-
 ?
2.4.1.10 sucrose + D-maltose
-
 Rahnella aquatilis JCM -1683 ?
-
 ?
2.4.1.10 sucrose + D-sorbitol
-
 Rahnella aquatilis ?
-
 ?
2.4.1.10 sucrose + D-sorbitol
-
 Rahnella aquatilis JCM -1683 ?
-
 ?
2.4.1.10 sucrose + D-xylose
-
 Rahnella aquatilis ?
-
 ?
2.4.1.10 sucrose + D-xylose
-
 Rahnella aquatilis JCM -1683 ?
-
 ?
2.4.1.10 sucrose + L-arabinose
-
 Rahnella aquatilis ?
-
 ?
2.4.1.10 sucrose + lactose
-
 Rahnella aquatilis ?
-
 ?
2.4.1.10 sucrose + lactose
-
 Rahnella aquatilis JCM -1683 ?
-
 ?
2.4.1.10 sucrose + maltotriose
-
 Rahnella aquatilis ?
-
 ?
2.4.1.10 sucrose + melibiose
-
 Rahnella aquatilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 dimer 2 * 64000, SDS-PAGE Rahnella aquatilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 30
-
 assay at Rahnella aquatilis
2.4.1.10 55 60 sucrose + D-xylose Rahnella aquatilis
2.4.1.10 60
-
-
 Rahnella aquatilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.10 40
-
 20 min, stable below Rahnella aquatilis
2.4.1.10 60
-
 20 min, 50% loss of activity Rahnella aquatilis
2.4.1.10 70
-
 20 min, complete loss of activity Rahnella aquatilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 6
-
-
 Rahnella aquatilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.10 3 10 almost no activity under pH 3.0 and above pH 10.0 Rahnella aquatilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.10 5.5 9 30°C, 2 h, stable Rahnella aquatilis