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Literature summary extracted from

  • Yanase, H.; Iwata, M.; Nakahigashi, R.; Kita, K.; Kato, N.; Tonomura, K.
    Purification, crystallization, and properties of the extracellular levansucrase from Zymomonas mobilis (1992), Biosci. Biotechnol. Biochem., 56, 1335-1337.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.10 crystallization from saturated (NH4)2SO4-solution, pH 6.0, 4°C, 20 mM potassium phosphate, 0.2 M NaCl Zymomonas mobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.10 Ag+
-
Zymomonas mobilis
2.4.1.10 Cu2+
-
Zymomonas mobilis
2.4.1.10 Hg2+
-
Zymomonas mobilis
2.4.1.10 Mersalyl acid
-
Zymomonas mobilis
2.4.1.10 additional information not: EDTA Zymomonas mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.10 122
-
sucrose
-
Zymomonas mobilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.10 extracellular
-
Zymomonas mobilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 56000
-
2 * 56000, SDS-PAGE Zymomonas mobilis
2.4.1.10 94000
-
gel filtration Zymomonas mobilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Zymomonas mobilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10
-
Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n at lower temperatures, such as 5°C and 15°C and pH 4.0-6.0 the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C and pH 7.0-8.0 the hydrolysis is preferentially catalyzed Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1
-
r
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n enzyme catalyzes the liberation of reducing sugars from substrates having 2-beta-D-fructofuranose residues on a terminal such as sucrose, raffinose, levan from Aerobacter levanicum and inulin with a relative activity of 100:104:1:0.01 Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1
-
r
2.4.1.10 sucrose + H2O hydrolysis Zymomonas mobilis D-glucose + D-fructose
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 dimer 2 * 56000, SDS-PAGE Zymomonas mobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 additional information
-
at lower temperatures, such as 4°C and 15°C, the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C the hydrolysis is preferentially catalyzed Zymomonas mobilis
2.4.1.10 30
-
assay at Zymomonas mobilis
2.4.1.10 50
-
-
Zymomonas mobilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.10 37
-
pH 6.0, 30 min Zymomonas mobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 4 6
-
Zymomonas mobilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.10 additional information
-
15°C, 10% sucrose, pH 4.0-6.0: transfructosylation preferentially occurs and oligo- and polysaccharides are produced, pH 7.0-8.0: hydrolysis of sucrose is enhanced, oligosaccharides are produced rather than polysaccharides as transfructosylation products Zymomonas mobilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.10 3.5 6 30°C, 24 h, stable Zymomonas mobilis