EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | 5'-AMP | activation | Rattus norvegicus | |
2.4.1.1 | 5'-AMP | half-maximal activation of phosphorylase Ib at 0.022 mM, 0.007 mM for phosphorylase IIb | Rattus norvegicus | |
2.4.1.1 | phosphate | activation | Rattus norvegicus | |
2.4.1.1 | phosphate | converts phosphorylase b into phosphorylase a | Rattus norvegicus | |
2.4.1.1 | Phosphorylase kinase | activation | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | Caffeine | 5 mM, 95% inhibition of heart phosphorylase Ib in the presence of 1 mM AMP, 30% inhibition of heart phosphorylase IIb | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | Rattus norvegicus | glycogen metabolism | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.1 | Rattus norvegicus | - |
male Wistar | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.4.1.1 | side-chain modification | incorporation of approx. 1 mol phosphate/mol of subunit | Rattus norvegicus |
2.4.1.1 | side-chain modification | activation by phosphorylation | Rattus norvegicus |
2.4.1.1 | side-chain modification | phosphorylation converts inactive phosphorylase b into active phosphorylase a | Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.1 | heart isozymes I and II, ammonium sulfate, DEAE-Sephacel, AMP-Sepharose, muscle enzyme, ammonium sulfate, DEAE-Sephacel | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.1 | heart | - |
Rattus norvegicus | - |
2.4.1.1 | muscle | skeletal muscle | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 46 | - |
heart isoenzymes I and II | Rattus norvegicus |
2.4.1.1 | 51.6 | - |
skeletal muscle | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.1 | -20°C, 50% glycerol, at least 4 months | Rattus norvegicus |
2.4.1.1 | glycerol stabilizes purified enzyme during storage | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | glycogen + glucose 1-phosphate | - |
Rattus norvegicus | glycogen + phosphate | - |
r | |
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Rattus norvegicus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | glycogen metabolism | Rattus norvegicus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.1 | dimer | SDS-PAGE | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | additional information | - |
pI: 6.2, skeletal muscle, heart isozyme Ib | Rattus norvegicus |
2.4.1.1 | additional information | - |
pI: 5.2, heart isozyme IIb | Rattus norvegicus |