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Literature summary extracted from

  • Berndt, N.; Rosen, P.
    Isolation and partial characterization of two forms of rat heart glycogen phosphorylase (1984), Arch. Biochem. Biophys., 228, 143-154.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 5'-AMP activation Rattus norvegicus
2.4.1.1 5'-AMP half-maximal activation of phosphorylase Ib at 0.022 mM, 0.007 mM for phosphorylase IIb Rattus norvegicus
2.4.1.1 phosphate activation Rattus norvegicus
2.4.1.1 phosphate converts phosphorylase b into phosphorylase a Rattus norvegicus
2.4.1.1 Phosphorylase kinase activation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 Caffeine 5 mM, 95% inhibition of heart phosphorylase Ib in the presence of 1 mM AMP, 30% inhibition of heart phosphorylase IIb Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate Rattus norvegicus glycogen metabolism [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Rattus norvegicus
-
 male Wistar
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.1 side-chain modification incorporation of approx. 1 mol phosphate/mol of subunit Rattus norvegicus
2.4.1.1 side-chain modification activation by phosphorylation Rattus norvegicus
2.4.1.1 side-chain modification phosphorylation converts inactive phosphorylase b into active phosphorylase a Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 heart isozymes I and II, ammonium sulfate, DEAE-Sephacel, AMP-Sepharose, muscle enzyme, ammonium sulfate, DEAE-Sephacel Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 heart
-
 Rattus norvegicus
-
2.4.1.1 muscle skeletal muscle Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 46
-
 heart isoenzymes I and II Rattus norvegicus
2.4.1.1 51.6
-
 skeletal muscle Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 -20°C, 50% glycerol, at least 4 months Rattus norvegicus
2.4.1.1 glycerol stabilizes purified enzyme during storage Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 glycogen + glucose 1-phosphate
-
 Rattus norvegicus glycogen + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Rattus norvegicus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate glycogen metabolism Rattus norvegicus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 additional information
-
 pI: 6.2, skeletal muscle, heart isozyme Ib Rattus norvegicus
2.4.1.1 additional information
-
 pI: 5.2, heart isozyme IIb Rattus norvegicus