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Literature summary extracted from

  • Nakamura, Y.; Imamura, M.
    Characterization of Chlorella phosphorylase: the glucan specificity and effect of temperature (1983), Phytochemistry, 22, 2395-2399.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 ADPglucose kinetics Chlorella vulgaris
2.4.1.1 additional information not inhibited by maltose, maltotriose, maltotetraose Chlorella vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information kinetic data Chlorella vulgaris
2.4.1.1 additional information
-
 additional information amylopectin, 0.69 mg/ml and 0.68 mg/ml for phosphorolysis and synthesis, respectively Chlorella vulgaris
2.4.1.1 0.21
-
 maltodecaheptaose phosphorolysis Chlorella vulgaris
2.4.1.1 0.5
-
 maltodecaheptaose synthesis Chlorella vulgaris
2.4.1.1 0.68
-
 maltohexaose phosphorolysis Chlorella vulgaris
2.4.1.1 0.72
-
 maltooctaose phosphorolysis Chlorella vulgaris
2.4.1.1 0.93
-
 maltopentaose phosphorolysis Chlorella vulgaris
2.4.1.1 1.22
-
 maltoheptaose phosphorolysis Chlorella vulgaris
2.4.1.1 2
-
 maltooctaose synthesis Chlorella vulgaris
2.4.1.1 2 8.3 maltooctaose maltoheptaose, maltohexaose, maltopentaose, maltotetraose, synthesis Chlorella vulgaris
2.4.1.1 2.2
-
 maltoheptaose synthesis Chlorella vulgaris
2.4.1.1 2.5
-
 maltohexaose synthesis Chlorella vulgaris
2.4.1.1 3
-
 maltopentaose synthesis Chlorella vulgaris
2.4.1.1 8.3
-
 maltotetraose synthesis Chlorella vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Chlorella vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 partial Chlorella vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (maltodextrin)n-1 + glucose 1-phosphate phosphorolysis activity ceases when maltodextrins are degraded to maltotetraose Chlorella vulgaris (maltodextrin)n + phosphate
-
 r
2.4.1.1 amylopectin + glucose 1-phosphate best substrate Chlorella vulgaris amylopectin + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate rabbit liver glycogen Chlorella vulgaris glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate glycogen from oyster Chlorella vulgaris glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate glycogen from: rabbit liver Chlorella vulgaris glycogen + phosphate
-
 r
2.4.1.1 maltodecaheptaose + phosphate
-
 Chlorella vulgaris maltodecahexaose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 maltoheptadecaose + glucose 1-phosphate
-
 Chlorella vulgaris maltooctadecaose + phosphate
-
 r
2.4.1.1 maltoheptaose + glucose 1-phosphate
-
 Chlorella vulgaris maltooctaose + phosphate
-
 r
2.4.1.1 maltohexaose + glucose 1-phosphate
-
 Chlorella vulgaris maltoheptaose + phosphate
-
 r
2.4.1.1 maltooctaose + glucose 1-phosphate
-
 Chlorella vulgaris maltononaose + phosphate
-
 r
2.4.1.1 maltopentaose + glucose 1-phosphate
-
 Chlorella vulgaris maltohexaose + phosphate
-
 r
2.4.1.1 maltotetraose + glucose 1-phosphate best substrate Chlorella vulgaris maltopentaose + phosphate
-
 ir
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate ineffective primer: glucose Chlorella vulgaris [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate polyglucose primer required Chlorella vulgaris [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 additional information
-
 ratio of phosphorolysis to synthesis increases significantly at higher temperatures Chlorella vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 5.1 6.8 synthesis, 2-(N-morpholino)ethanesulfonic acid buffer Chlorella vulgaris
2.4.1.1 7 7.5 phosphorolysis Chlorella vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 5.6 7.5 approx. half-maximal activity at pH 5.6 and pH 7.5, imidazole buffer, phosphorolysis Chlorella vulgaris
2.4.1.1 7 8.1 maximal activity at pH 7, approx. 60% of maximal activity at pH 8.1, glycylglycine buffer, phosphorolysis Chlorella vulgaris