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Literature summary extracted from

  • Tanabe, S.; Kobayashi, M.; Matsuda, K.
    Yeast glycogen phosphorylase: characterization of the dimeric form and its activation (1987), Agric. Biol. Chem., 51, 2465-2471.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 phosphate activation Saccharomyces cerevisiae
2.4.1.1 phosphate phosphorylation site: a single Thr-residue in the N-terminal region Saccharomyces cerevisiae
2.4.1.1 Phosphorylase kinase activation Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 AMP 10 mM, 55% inhibition, competitive to glucose 1-phosphate Saccharomyces cerevisiae
2.4.1.1 Caffeine weak Saccharomyces cerevisiae
2.4.1.1 Cu2+ 4 mM, more than 50% inhibition Saccharomyces cerevisiae
2.4.1.1 cyclodextrin alpha- or beta-, not gamma-cyclodextrin; i.e. structure analogue of alpha-1,4-linked starch molecule Saccharomyces cerevisiae
2.4.1.1 glucose 6-phosphate 5 mM, 54% inhibition Saccharomyces cerevisiae
2.4.1.1 Hg2+ 4 mM, more than 50% inhibition Saccharomyces cerevisiae
2.4.1.1 additional information not inhibited by IMP Saccharomyces cerevisiae
2.4.1.1 NaF 200 mM, complete inhibition Saccharomyces cerevisiae
2.4.1.1 UDPglucose 5 mM, 42% inhibition Saccharomyces cerevisiae
2.4.1.1 Zn2+ 4 mM, more than 50% inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 100000
-
 2 * 100000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.1 side-chain modification activation by phosphorylation Saccharomyces cerevisiae
2.4.1.1 side-chain modification phosphorylation site: a single Thr-residue in the N-terminal region Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 Streptomycin, 33% ethanol, DEAE-Sephadex, glycogen-Sepharose Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 42.3
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 glycogen + glucose 1-phosphate
-
 Saccharomyces cerevisiae glycogen + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Saccharomyces cerevisiae [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 100000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 30
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 25
-
 stable below Saccharomyces cerevisiae
2.4.1.1 40 50 inactivation Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 5.8
-
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.1 5 8 stable Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 9.3
-
 AMP
-
 Saccharomyces cerevisiae