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Literature summary extracted from

  • Rath, V.L.; Hwang, P.K.; Fletterick, R.J.
    Purification and crystallization of glycogen phosphorylase from Saccharomyces cerevisiae (1992), J. Mol. Biol., 225, 1027-1034.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 phosphate activation Saccharomyces cerevisiae
2.4.1.1 phosphate yeast and mammalian enzymes are activated by covalent phosphorylation, phosphorylase kinase Saccharomyces cerevisiae
2.4.1.1 phosphate phosphorylation site: a single Thr-residue in the N-terminal region Saccharomyces cerevisiae
2.4.1.1 Phosphorylase kinase activation Saccharomyces cerevisiae
2.4.1.1 Phosphorylase kinase yeast and mammalian enzymes are activated by covalent phosphorylation of a single Thr-residue in the N-terminal region Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.1 hanging-drop vapor diffusion, crystals diffract to 2.4 A resolution Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 glucose 6-phosphate above 5 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Saccharomyces cerevisiae
-
 harbouring plasmid Yep24::GPH1
-
2.4.1.1 Saccharomyces cerevisiae PH5-3
-
 harbouring plasmid Yep24::GPH1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.1 side-chain modification activation by phosphorylation Saccharomyces cerevisiae
2.4.1.1 side-chain modification phosphorylation site: a single Thr-residue in the N-terminal region Saccharomyces cerevisiae
2.4.1.1 side-chain modification yeast and mammalian enzymes are activated by covalent phosphorylation, phosphorylase kinase Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 4.7
-
 non-phosphorylated enzyme Saccharomyces cerevisiae
2.4.1.1 85 90 phosphorylated enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 glycogen + glucose 1-phosphate favoured reaction Saccharomyces cerevisiae glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate favoured reaction Saccharomyces cerevisiae PH5-3 glycogen + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Saccharomyces cerevisiae [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Saccharomyces cerevisiae PH5-3 [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r