EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | 5'-AMP | activation | Dictyostelium discoideum | |
2.4.1.1 | 5'-AMP | half-maximal activation of isoenzyme b at approx. 0.7 mM, isozyme a is not activated | Dictyostelium discoideum |
EC Number | General Stability | Organism |
---|---|---|
2.4.1.1 | freeze-thawing inactivates | Dictyostelium discoideum |
2.4.1.1 | Streptomycin sulfate or ammonium sulfate precipitation inactivates b-, not a-isozyme | Dictyostelium discoideum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | AMP | isozyme a; weak | Dictyostelium discoideum | |
2.4.1.1 | high salt concentration | - |
Dictyostelium discoideum | |
2.4.1.1 | KCl | 80 mM, 50% loss of activity, 200 mM, 90% loss of activity | Dictyostelium discoideum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | additional information | - |
additional information | 2.7 mg/ml, glycogen, isozyme a | Dictyostelium discoideum | |
2.4.1.1 | additional information | - |
additional information | 4.2 mg/ml, glycogen, isozyme b | Dictyostelium discoideum | |
2.4.1.1 | 1.2 | - |
phosphate | isoenzyme A | Dictyostelium discoideum | |
2.4.1.1 | 1.5 | - |
phosphate | isoenzyme b, in the presence of 3 mM 5'-AMP | Dictyostelium discoideum | |
2.4.1.1 | 55 | - |
phosphate | isoenzyme b, in the absence of 5'-AMP | Dictyostelium discoideum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.1 | cytoplasm | - |
Dictyostelium discoideum | 5737 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | Dictyostelium discoideum | key step in cellular differentiation of Dictyostelium discoideum | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.1 | Dictyostelium discoideum | - |
slime mould, strain Ax3 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.1 | isozymes a, streptomycin sulfate, ammonium sulfate, 50°C, DE52-cellulose, aminobutyl-agarose, Sephacryl S-300, isoenzyme b, DE52-cellulose, 5'-AMP-Sepharose | Dictyostelium discoideum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 3.3 | - |
isozyme B | Dictyostelium discoideum |
2.4.1.1 | 4.3 | - |
isozyme A | Dictyostelium discoideum |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.1 | 7°C, isozyme b, inactivation within 48 h, isozyme a: at least 48 h, in crude extract | Dictyostelium discoideum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | glycogen + glucose 1-phosphate | rabbit liver glycogen | Dictyostelium discoideum | glycogen + phosphate | - |
? | |
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Dictyostelium discoideum | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | key step in cellular differentiation of Dictyostelium discoideum | Dictyostelium discoideum | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.1 | dimer | SDS-PAGE | Dictyostelium discoideum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 26 | - |
- |
Dictyostelium discoideum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 18 | 35 | approx. half-maximal activity at 18°C and 35°C | Dictyostelium discoideum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 42 | - |
inactivation above | Dictyostelium discoideum |
2.4.1.1 | 50 | - |
inactivation within 5 min, isozyme b | Dictyostelium discoideum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 6.6 | - |
isozyme a, 2-(N-morpholino)ethanesulfonic acid preferred to imidazole buffer | Dictyostelium discoideum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 5.8 | 7.6 | approx. half-maximal activity at pH 5.8 and 7.6, b-isozyme | Dictyostelium discoideum |
2.4.1.1 | 5.8 | 7.2 | approx. half-maximal activity at pH 5.8 and 7.2, a-isozyme | Dictyostelium discoideum |