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Literature summary extracted from

  • Cloutier, M.J.; Rutherford, C.L.
    Glycogen phosphorylase in Dictyostelium. Developmental regulation of two forms and their physical and kinetic properties (1987), J. Biol. Chem., 262, 9486-9493.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 5'-AMP activation Dictyostelium discoideum
2.4.1.1 5'-AMP half-maximal activation of isoenzyme b at approx. 0.7 mM, isozyme a is not activated Dictyostelium discoideum

General Stability

EC Number General Stability Organism
2.4.1.1 freeze-thawing inactivates Dictyostelium discoideum
2.4.1.1 Streptomycin sulfate or ammonium sulfate precipitation inactivates b-, not a-isozyme Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 AMP isozyme a; weak Dictyostelium discoideum
2.4.1.1 high salt concentration
-
 Dictyostelium discoideum
2.4.1.1 KCl 80 mM, 50% loss of activity, 200 mM, 90% loss of activity Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information 2.7 mg/ml, glycogen, isozyme a Dictyostelium discoideum
2.4.1.1 additional information
-
 additional information 4.2 mg/ml, glycogen, isozyme b Dictyostelium discoideum
2.4.1.1 1.2
-
 phosphate isoenzyme A Dictyostelium discoideum
2.4.1.1 1.5
-
 phosphate isoenzyme b, in the presence of 3 mM 5'-AMP Dictyostelium discoideum
2.4.1.1 55
-
 phosphate isoenzyme b, in the absence of 5'-AMP Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 cytoplasm
-
 Dictyostelium discoideum 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate Dictyostelium discoideum key step in cellular differentiation of Dictyostelium discoideum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Dictyostelium discoideum
-
 slime mould, strain Ax3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 isozymes a, streptomycin sulfate, ammonium sulfate, 50°C, DE52-cellulose, aminobutyl-agarose, Sephacryl S-300, isoenzyme b, DE52-cellulose, 5'-AMP-Sepharose Dictyostelium discoideum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 3.3
-
 isozyme B Dictyostelium discoideum
2.4.1.1 4.3
-
 isozyme A Dictyostelium discoideum

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 7°C, isozyme b, inactivation within 48 h, isozyme a: at least 48 h, in crude extract Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 glycogen + glucose 1-phosphate rabbit liver glycogen Dictyostelium discoideum glycogen + phosphate
-
 ?
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Dictyostelium discoideum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate key step in cellular differentiation of Dictyostelium discoideum Dictyostelium discoideum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer SDS-PAGE Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 26
-
-
 Dictyostelium discoideum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.1 18 35 approx. half-maximal activity at 18°C and 35°C Dictyostelium discoideum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 42
-
 inactivation above Dictyostelium discoideum
2.4.1.1 50
-
 inactivation within 5 min, isozyme b Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6.6
-
 isozyme a, 2-(N-morpholino)ethanesulfonic acid preferred to imidazole buffer Dictyostelium discoideum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 5.8 7.6 approx. half-maximal activity at pH 5.8 and 7.6, b-isozyme Dictyostelium discoideum
2.4.1.1 5.8 7.2 approx. half-maximal activity at pH 5.8 and 7.2, a-isozyme Dictyostelium discoideum