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Literature summary extracted from

  • Thomas, D.A.; Wright, B.E.
    Glycogen phosphorylase in Dictyostelium discoideum. I. Purification and properties of the enzyme (1976), J. Biol. Chem., 254, 1253-1257.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 additional information not activated by ATP, cAMP Dictyostelium discoideum
2.4.1.1 additional information not activated by ATP-regenerating systems Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 ADPglucose competitive inhibition Dictyostelium discoideum
2.4.1.1 GDPglucose 0.05 mM, 50% inhibition, competitive inhibition of phosphorolysis Dictyostelium discoideum
2.4.1.1 glucose 1-phosphate kinetics Dictyostelium discoideum
2.4.1.1 UDPglucose kinetics Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information 5 mg/ml, oyster glycogen Dictyostelium discoideum
2.4.1.1 3.6
-
 phosphate
-
 Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 95000
-
 2 * 95000 Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Dictyostelium discoideum
-
 slime mould, strain NC-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 Streptomycin sulfate, ammonium sulfate, heat, DEAE-cellulose, Sepharose 2B, preparative gel electrophoresis Dictyostelium discoideum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 7.14
-
-
 Dictyostelium discoideum

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 glycerol stabilizes purified enzyme during storage Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 glycogen + glucose 1-phosphate glycogen from: oyster Dictyostelium discoideum glycogen + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Dictyostelium discoideum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 95000 Dictyostelium discoideum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 0.1
-
 glucose 1-phosphate
-
 Dictyostelium discoideum