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Literature summary extracted from

  • Nader, W.; Becker, J.U.
    1,4-alpha-Glucan phosphorylase from the slime mold Physarum polycephalum. Purification, physico-chemical and kinetic properties (1979), Eur. J. Biochem., 102, 345-355.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 5'-AMP activation Badhamia polycephala
2.4.1.1 5'-AMP slight activation Badhamia polycephala

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 D-glucose weak Badhamia polycephala
2.4.1.1 fructose 1-phosphate
-
 Badhamia polycephala
2.4.1.1 fructose 6-phosphate
-
 Badhamia polycephala
2.4.1.1 GDPglucose kinetics Badhamia polycephala
2.4.1.1 glucose 1-phosphate completely reversible by 5'-AMP; kinetics Badhamia polycephala
2.4.1.1 glucose 6-phosphate
-
 Badhamia polycephala

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information kinetic data Badhamia polycephala
2.4.1.1 additional information
-
 additional information 1.26 mg/ml, glycogen Badhamia polycephala

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 93000
-
 2 * 93000, SDS-PAGE Badhamia polycephala
2.4.1.1 172000
-
 gel filtration Badhamia polycephala

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Badhamia polycephala
-
 M3c
-
2.4.1.1 Badhamia polycephala M3c
-
 M3c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 Streptomycin, ammonium sulfate, DEAE-cellulose, aminobutyl-Sepharose, hydroxyapatite Badhamia polycephala

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 plasmodium macroplasmodium Badhamia polycephala
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 4.2
-
-
 Badhamia polycephala

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 6°C, 3 months Badhamia polycephala
2.4.1.1 bovine serum albumin prevents surface inactivation during storage Badhamia polycephala

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 glycogen + glucose 1-phosphate glycogen from oyster Badhamia polycephala glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate glycogen from Streptococcus salivarius Badhamia polycephala glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate glycogen from oyster Badhamia polycephala M3c glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate glycogen from Streptococcus salivarius Badhamia polycephala M3c glycogen + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Badhamia polycephala [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Badhamia polycephala M3c [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 93000, SDS-PAGE Badhamia polycephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6 7.5 broad Badhamia polycephala

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 5.4 8.2 approx. half-maximal activity at pH 5.4 and pH 8.2 Badhamia polycephala

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.1 pyridoxal 5'-phosphate requirement Badhamia polycephala
2.4.1.1 pyridoxal 5'-phosphate 1 mol/mol subunit Badhamia polycephala

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 0.03
-
 GDP-glucose
-
 Badhamia polycephala
2.4.1.1 0.1
-
 glucose 1-phosphate
-
 Badhamia polycephala