EC Number | General Stability | Organism |
---|---|---|
2.4.1.1 | phosphate protects against thermal inactivation | Klebsiella pneumoniae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | additional information | not inhibited by AMP | Klebsiella pneumoniae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 88000 | - |
2 * 88000, SDS-PAGE | Klebsiella pneumoniae |
2.4.1.1 | 90000 | - |
2 * 90000, low speed sedimentation equilibrium, after treatment with 6 M guanidine-HCl | Klebsiella pneumoniae |
2.4.1.1 | 174000 | - |
high speed sedimentation equilibrium | Klebsiella pneumoniae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.1 | Klebsiella pneumoniae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.1 | ammonium sulfate, polyethylenglycol, DEAE-cellulose, CM-Sephadex | Klebsiella pneumoniae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 17.7 | - |
maltoheptaose | Klebsiella pneumoniae |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.1 | 4°C, 3.5 M (NH4)2SO4 in solution of neutral pH-value, several months, no loss of activity | Klebsiella pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | (maltodextrin)n-1 + glucose 1-phosphate | glucosylation at 96% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | (maltodextrin)n + phosphate | - |
r | |
2.4.1.1 | amylopectin + glucose 1-phosphate | glucosylation at 23% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | amylopectin + phosphate | - |
r | |
2.4.1.1 | amylopectin + glucose 1-phosphate | waxy maize amylopectin | Klebsiella pneumoniae | amylopectin + phosphate | - |
r | |
2.4.1.1 | amylose + glucose 1-phosphate | glucosylation at 7% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | amylose + phosphate | - |
? | |
2.4.1.1 | glycogen + glucose 1-phosphate | glycogen from: rabbit liver | Klebsiella pneumoniae | glycogen + phosphate | - |
r | |
2.4.1.1 | glycogen + glucose 1-phosphate | glucosylation at 14.4% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | glycogen + phosphate | - |
r | |
2.4.1.1 | maltoheptaose + glucose 1-phosphate | - |
Klebsiella pneumoniae | maltooctaose + phosphate | - |
r | |
2.4.1.1 | maltotetraose + glucose 1-phosphate | glucosylation at 22% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | maltopentaose + phosphate | - |
r | |
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | no activity with pullulan | Klebsiella pneumoniae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | no activity with dextran | Klebsiella pneumoniae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.1 | dimer | 2 * 88000, SDS-PAGE | Klebsiella pneumoniae |
2.4.1.1 | dimer | 2 * 90000, low speed sedimentation equilibrium, after treatment with 6 M guanidine-HCl | Klebsiella pneumoniae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | additional information | - |
phosphate protects against thermal inactivation | Klebsiella pneumoniae |
2.4.1.1 | 30 | - |
30 min stable at pH 5.9-10.5 | Klebsiella pneumoniae |
2.4.1.1 | 43 | - |
10 min stable | Klebsiella pneumoniae |
2.4.1.1 | 55 | - |
loss of approx. 90% activity after 10 min in 500 mM potassium phosphate pH 7.3 | Klebsiella pneumoniae |
2.4.1.1 | 63 | - |
inactivation within 10 min | Klebsiella pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | additional information | - |
pI: 5.3 | Klebsiella pneumoniae |
2.4.1.1 | 5.9 | - |
- |
Klebsiella pneumoniae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 5.1 | 7.5 | approx. half-maximal activity at pH 5.1 and 7.5 | Klebsiella pneumoniae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 5.9 | 10.5 | 30 min stable at 30°C | Klebsiella pneumoniae |
2.4.1.1 | 11.5 | - |
and above, denaturation | Klebsiella pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | pyridoxal 5'-phosphate | 2 mol per mol enzyme | Klebsiella pneumoniae |