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Literature summary extracted from

  • Linder, D.; Kurz, G.; Bender, H.; Wallenfels, K.
    1,4-alpha-Glucan phosphorylase from Klebsiella pneumoniae purification, subunit structure and amino acid composition (1976), Eur. J. Biochem., 70, 291-303.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.1 phosphate protects against thermal inactivation Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 additional information not inhibited by AMP Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 88000
-
2 * 88000, SDS-PAGE Klebsiella pneumoniae
2.4.1.1 90000
-
2 * 90000, low speed sedimentation equilibrium, after treatment with 6 M guanidine-HCl Klebsiella pneumoniae
2.4.1.1 174000
-
high speed sedimentation equilibrium Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 ammonium sulfate, polyethylenglycol, DEAE-cellulose, CM-Sephadex Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 17.7
-
maltoheptaose Klebsiella pneumoniae

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 4°C, 3.5 M (NH4)2SO4 in solution of neutral pH-value, several months, no loss of activity Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (maltodextrin)n-1 + glucose 1-phosphate glucosylation at 96% the rate of maltoheptaose glucosylation Klebsiella pneumoniae (maltodextrin)n + phosphate
-
r
2.4.1.1 amylopectin + glucose 1-phosphate glucosylation at 23% the rate of maltoheptaose glucosylation Klebsiella pneumoniae amylopectin + phosphate
-
r
2.4.1.1 amylopectin + glucose 1-phosphate waxy maize amylopectin Klebsiella pneumoniae amylopectin + phosphate
-
r
2.4.1.1 amylose + glucose 1-phosphate glucosylation at 7% the rate of maltoheptaose glucosylation Klebsiella pneumoniae amylose + phosphate
-
?
2.4.1.1 glycogen + glucose 1-phosphate glycogen from: rabbit liver Klebsiella pneumoniae glycogen + phosphate
-
r
2.4.1.1 glycogen + glucose 1-phosphate glucosylation at 14.4% the rate of maltoheptaose glucosylation Klebsiella pneumoniae glycogen + phosphate
-
r
2.4.1.1 maltoheptaose + glucose 1-phosphate
-
Klebsiella pneumoniae maltooctaose + phosphate
-
r
2.4.1.1 maltotetraose + glucose 1-phosphate glucosylation at 22% the rate of maltoheptaose glucosylation Klebsiella pneumoniae maltopentaose + phosphate
-
r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate no activity with pullulan Klebsiella pneumoniae [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate no activity with dextran Klebsiella pneumoniae [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 88000, SDS-PAGE Klebsiella pneumoniae
2.4.1.1 dimer 2 * 90000, low speed sedimentation equilibrium, after treatment with 6 M guanidine-HCl Klebsiella pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 additional information
-
phosphate protects against thermal inactivation Klebsiella pneumoniae
2.4.1.1 30
-
30 min stable at pH 5.9-10.5 Klebsiella pneumoniae
2.4.1.1 43
-
10 min stable Klebsiella pneumoniae
2.4.1.1 55
-
loss of approx. 90% activity after 10 min in 500 mM potassium phosphate pH 7.3 Klebsiella pneumoniae
2.4.1.1 63
-
inactivation within 10 min Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 additional information
-
pI: 5.3 Klebsiella pneumoniae
2.4.1.1 5.9
-
-
Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 5.1 7.5 approx. half-maximal activity at pH 5.1 and 7.5 Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.1 5.9 10.5 30 min stable at 30°C Klebsiella pneumoniae
2.4.1.1 11.5
-
and above, denaturation Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.1 pyridoxal 5'-phosphate 2 mol per mol enzyme Klebsiella pneumoniae