BRENDA - Enzyme Database

Properties of methylcitrate synthase from Candida lipolytica

Uchiyama, H.; Tabuchi, T.; Agric. Biol. Chem. 40, 1411-1418 (1976)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.3.5
isocitrate
1 mM, relative activity 101%
Yarrowia lipolytica
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.3.5
ADP
slight inhibition
Yarrowia lipolytica
2.3.3.5
ATP
-
Yarrowia lipolytica
2.3.3.5
H2O2
-
Yarrowia lipolytica
2.3.3.5
Hg2+
-
Yarrowia lipolytica
2.3.3.5
Monoiodoacetamide
-
Yarrowia lipolytica
2.3.3.5
NADH
-
Yarrowia lipolytica
2.3.3.5
NADPH
-
Yarrowia lipolytica
2.3.3.5
p-chloromercuribenzoate
-
Yarrowia lipolytica
2.3.3.5
Zn2+
-
Yarrowia lipolytica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.3.5
0.0029
-
acetyl-CoA
-
Yarrowia lipolytica
2.3.3.5
0.0053
-
propionyl-CoA
-
Yarrowia lipolytica
2.3.3.5
0.022
-
butyryl-CoA
-
Yarrowia lipolytica
2.3.3.5
0.025
-
oxaloacetate
-
Yarrowia lipolytica
2.3.3.5
0.025
-
valeryl-CoA
-
Yarrowia lipolytica
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.3.5
Ca2+
1 mM, relative activity 104%
Yarrowia lipolytica
2.3.3.5
Fe2+
0.02 mM, relative activity 104%
Yarrowia lipolytica
2.3.3.5
Mg2+
1 mM, relative activity 102%
Yarrowia lipolytica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
Yarrowia lipolytica
physiological role of the methylcitric acid cycle is a catabolic reaction sequence concerning the oxidation of propionate into pyruvate, regulatory enzyme of this cycle
2-methylcitrate + CoA
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
Escherichia coli
first key enzyme of the methylcitric acid cycle
2-methylcitrate + CoA
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
Yarrowia lipolytica
first key enzyme of the methylcitric acid cycle
2-methylcitrate + CoA
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.3.5
Escherichia coli
-
-
-
2.3.3.5
Yarrowia lipolytica
-
formerly Candida lipolytica; IFO 1659
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.3.5
-
Yarrowia lipolytica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.3.5
0.436
-
substrate propionyl-CoA
Yarrowia lipolytica
2.3.3.5
0.623
-
substrate acetyl-CoA
Yarrowia lipolytica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.3.5
acetyl-CoA + H2O + oxaloacetate
-
488166
Yarrowia lipolytica
citrate + CoA
-
-
-
?
2.3.3.5
additional information
enzyme is specific for oxaloacetate, does not catalyze liberation of CoA from acetyl- or propionyl-CoA in presence of glyoxylate, pyruvate or alpha-ketoglutarate, isobutyryl-CoA and isovaleryl-CoA are no substrates
488166
Yarrowia lipolytica
?
-
-
-
-
2.3.3.5
n-butyryl-CoA + H2O + oxaloacetate
-
488166
Yarrowia lipolytica
2-ethylcitrate + CoA
-
-
-
r
2.3.3.5
n-valeryl-CoA + H2O + oxaloacetate
-
488166
Yarrowia lipolytica
2-propylcitrate + CoA
-
-
-
r
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
-
488166
Escherichia coli
2-methylcitrate + CoA
-
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
-
488166
Yarrowia lipolytica
2-methylcitrate + CoA
-
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
physiological role of the methylcitric acid cycle is a catabolic reaction sequence concerning the oxidation of propionate into pyruvate, regulatory enzyme of this cycle
488166
Yarrowia lipolytica
2-methylcitrate + CoA
-
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
first key enzyme of the methylcitric acid cycle
488166
Escherichia coli
2-methylcitrate + CoA
-
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
first key enzyme of the methylcitric acid cycle
488166
Yarrowia lipolytica
2-methylcitrate + CoA
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.3.5
45
-
-
Yarrowia lipolytica
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.3.5
10
50
-
Yarrowia lipolytica
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.3.5
50
-
activity completely lost after 10 min
Yarrowia lipolytica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.3.5
8
8.5
-
Yarrowia lipolytica
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.3.5
6
9.5
-
Yarrowia lipolytica
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.3.5
isocitrate
1 mM, relative activity 101%
Yarrowia lipolytica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.3.5
ADP
slight inhibition
Yarrowia lipolytica
2.3.3.5
ATP
-
Yarrowia lipolytica
2.3.3.5
H2O2
-
Yarrowia lipolytica
2.3.3.5
Hg2+
-
Yarrowia lipolytica
2.3.3.5
Monoiodoacetamide
-
Yarrowia lipolytica
2.3.3.5
NADH
-
Yarrowia lipolytica
2.3.3.5
NADPH
-
Yarrowia lipolytica
2.3.3.5
p-chloromercuribenzoate
-
Yarrowia lipolytica
2.3.3.5
Zn2+
-
Yarrowia lipolytica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.3.5
0.0029
-
acetyl-CoA
-
Yarrowia lipolytica
2.3.3.5
0.0053
-
propionyl-CoA
-
Yarrowia lipolytica
2.3.3.5
0.022
-
butyryl-CoA
-
Yarrowia lipolytica
2.3.3.5
0.025
-
oxaloacetate
-
Yarrowia lipolytica
2.3.3.5
0.025
-
valeryl-CoA
-
Yarrowia lipolytica
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.3.5
Ca2+
1 mM, relative activity 104%
Yarrowia lipolytica
2.3.3.5
Fe2+
0.02 mM, relative activity 104%
Yarrowia lipolytica
2.3.3.5
Mg2+
1 mM, relative activity 102%
Yarrowia lipolytica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
Yarrowia lipolytica
physiological role of the methylcitric acid cycle is a catabolic reaction sequence concerning the oxidation of propionate into pyruvate, regulatory enzyme of this cycle
2-methylcitrate + CoA
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
Escherichia coli
first key enzyme of the methylcitric acid cycle
2-methylcitrate + CoA
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
Yarrowia lipolytica
first key enzyme of the methylcitric acid cycle
2-methylcitrate + CoA
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.3.5
-
Yarrowia lipolytica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.3.5
0.436
-
substrate propionyl-CoA
Yarrowia lipolytica
2.3.3.5
0.623
-
substrate acetyl-CoA
Yarrowia lipolytica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.3.5
acetyl-CoA + H2O + oxaloacetate
-
488166
Yarrowia lipolytica
citrate + CoA
-
-
-
?
2.3.3.5
additional information
enzyme is specific for oxaloacetate, does not catalyze liberation of CoA from acetyl- or propionyl-CoA in presence of glyoxylate, pyruvate or alpha-ketoglutarate, isobutyryl-CoA and isovaleryl-CoA are no substrates
488166
Yarrowia lipolytica
?
-
-
-
-
2.3.3.5
n-butyryl-CoA + H2O + oxaloacetate
-
488166
Yarrowia lipolytica
2-ethylcitrate + CoA
-
-
-
r
2.3.3.5
n-valeryl-CoA + H2O + oxaloacetate
-
488166
Yarrowia lipolytica
2-propylcitrate + CoA
-
-
-
r
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
-
488166
Escherichia coli
2-methylcitrate + CoA
-
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
-
488166
Yarrowia lipolytica
2-methylcitrate + CoA
-
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
physiological role of the methylcitric acid cycle is a catabolic reaction sequence concerning the oxidation of propionate into pyruvate, regulatory enzyme of this cycle
488166
Yarrowia lipolytica
2-methylcitrate + CoA
-
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
first key enzyme of the methylcitric acid cycle
488166
Escherichia coli
2-methylcitrate + CoA
-
-
-
?
2.3.3.5
propanoyl-CoA + H2O + oxaloacetate
first key enzyme of the methylcitric acid cycle
488166
Yarrowia lipolytica
2-methylcitrate + CoA
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.3.5
45
-
-
Yarrowia lipolytica
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.3.5
10
50
-
Yarrowia lipolytica
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.3.5
50
-
activity completely lost after 10 min
Yarrowia lipolytica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.3.5
8
8.5
-
Yarrowia lipolytica
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.3.5
6
9.5
-
Yarrowia lipolytica