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Literature summary extracted from

  • Mahlen, A.
    Properties of 2-decylcitrate synthase from Penicillium spiculisporum Lehman (1971), Eur. J. Biochem., 22, 104-114.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.2 5,5'-dithio-bis-(2-nitrobenzoic acid)
-
 Talaromyces trachyspermus
2.3.3.2 acetyl-CoA
-
 Talaromyces trachyspermus
2.3.3.2 CoASSCoA
-
 Talaromyces trachyspermus
2.3.3.2 iodoacetamide
-
 Talaromyces trachyspermus
2.3.3.2 lauroyl-CoA strong substrate inhibition, does not follow Michaelis-Menten kinetics Talaromyces trachyspermus
2.3.3.2 N-ethylmaleimide
-
 Talaromyces trachyspermus
2.3.3.2 NADH at 5 mM, slight inhibition Talaromyces trachyspermus
2.3.3.2 p-chloromercuribenzoate
-
 Talaromyces trachyspermus
2.3.3.2 palmitoyl-CoA
-
 Talaromyces trachyspermus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.2 0.0001
-
 lauroyl-CoA
-
 Talaromyces trachyspermus
2.3.3.2 0.03
-
 11-formamidoundecanoyl-CoA
-
 Talaromyces trachyspermus
2.3.3.2 0.035
-
 oxaloacetate at 0.021 mM lauroyl-CoA Talaromyces trachyspermus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.2 45000
-
 2 * 45000, SDS-PAGE Talaromyces trachyspermus
2.3.3.2 90000
-
 gel filtration Talaromyces trachyspermus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.2 lauroyl-CoA + H2O + oxaloacetate Talaromyces trachyspermus
-
 (2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
 ?
2.3.3.2 lauroyl-CoA + H2O + oxaloacetate Talaromyces trachyspermus Lehman
-
 (2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.2 Talaromyces trachyspermus
-
-
-
2.3.3.2 Talaromyces trachyspermus Lehman
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.2
-
 Talaromyces trachyspermus

Storage Stability

EC Number Storage Stability Organism
2.3.3.2 -18°C, 0.5 M (NH4)2SO4, 20% activity loss, 2 months Talaromyces trachyspermus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.2 11-formamidoundecanoyl-CoA + H2O + oxaloacetate
-
 Talaromyces trachyspermus (2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
-
 ?
2.3.3.2 11-formamidoundecanoyl-CoA + H2O + oxaloacetate
-
 Talaromyces trachyspermus Lehman (2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
-
 ?
2.3.3.2 lauroyl-CoA + H2O + oxaloacetate
-
 Talaromyces trachyspermus (2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
 ?
2.3.3.2 lauroyl-CoA + H2O + oxaloacetate
-
 Talaromyces trachyspermus Lehman (2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.2 dimer 2 * 45000, SDS-PAGE Talaromyces trachyspermus