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Literature summary extracted from

  • Cabano, J.; Buesa, C.; Hegardt, F.G.; Marrero, P.F.
    Catalytic properties of recombinant 3-hydroxy-3-methylglutaryl coenzyme A synthase-1 from Blattella germanica (1997), Insect Biochem. Mol. Biol., 27, 499-505.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10 2 cytosolic HMG-CoA synthase genes, HMGS-1 and HMGs-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S Blattella germanica
2.3.3.10 intronless gene, encodes for an active enzyme able to complement eukaryotic Mev-1 cells Blattella germanica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 acetoacetyl-CoA
-
Blattella germanica
2.3.3.10 acetoacetyl-CoA
-
Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.0001
-
acetoacetyl-CoA
-
Saccharomyces cerevisiae
2.3.3.10 0.014
-
acetyl-CoA
-
Saccharomyces cerevisiae
2.3.3.10 0.0152
-
acetyl-CoA
-
Blattella germanica
2.3.3.10 0.051
-
acetyl-CoA
-
Bos taurus
2.3.3.10 0.2
-
acetyl-CoA
-
Diploptera punctata
2.3.3.10 0.3
-
acetyl-CoA
-
Gallus gallus
2.3.3.10 0.4
-
acetyl-CoA
-
Locusta migratoria

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 cytosol
-
Gallus gallus 5829
-
2.3.3.10 cytosol
-
Saccharomyces cerevisiae 5829
-
2.3.3.10 cytosol
-
Blattella germanica 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 50000
-
SDS-PAGE, immunoblot by antibodies, prediction from cDNA nucleotide sequence Blattella germanica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae
-
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Bos taurus
-
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Locusta migratoria
-
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Diploptera punctata
-
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Blattella germanica regulation of isoprenoid synthesis and metabolism (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Blattella germanica
-
cockroach
-
2.3.3.10 Bos taurus
-
ox
-
2.3.3.10 Diploptera punctata
-
-
-
2.3.3.10 Gallus gallus
-
chicken
-
2.3.3.10 Locusta migratoria
-
-
-
2.3.3.10 Saccharomyces cerevisiae
-
baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10 recombinant enzyme Blattella germanica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 corpus allatum
-
Locusta migratoria
-
2.3.3.10 corpus allatum
-
Diploptera punctata
-
2.3.3.10 fat body
-
Blattella germanica
-
2.3.3.10 liver
-
Gallus gallus
-
2.3.3.10 liver
-
Bos taurus
-
2.3.3.10 ovary
-
Blattella germanica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 40
-
-
Blattella germanica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
Bos taurus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
Locusta migratoria (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
Blattella germanica (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
Diploptera punctata (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O regulation of isoprenoid synthesis and metabolism Blattella germanica (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
2.3.3.10 additional information
-
Gallus gallus ?
-
?
2.3.3.10 additional information
-
Saccharomyces cerevisiae ?
-
?
2.3.3.10 additional information first organism known to have 2 functional cytosolic HMG-CoA synthases Blattella germanica ?
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.10 0.00126
-
acetoacetyl-CoA
-
Blattella germanica
2.3.3.10 0.0035
-
acetoacetyl-CoA
-
Bos taurus