Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Misra, I.; Miziorko, H.M.
    Evidence for the interaction of avian 3-hydroxy-3-methylglutaryl-CoA synthase H264 with acetoacetyl-CoA (1996), Biochemistry, 35, 9610-9616.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.3.10 medicine target of a potent antisteroidogenic drug Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10 expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 3-carboxy-2,2,5,5,-tetramethyl-1-pyrrolidinyloxyl-CoA
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.00055
-
 acetoacetyl-CoA mutant H197N Gallus gallus
2.3.3.10 0.00062
-
 acetoacetyl-CoA mutant H436N Gallus gallus
2.3.3.10 0.00085
-
 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 0.015
-
 acetyl-CoA acetyl-CoA hydrolysis Gallus gallus
2.3.3.10 0.021
-
 S-(3-oxobutyl)-CoA
-
 Gallus gallus
2.3.3.10 0.036
-
 acetyl-CoA mutant H264A, acetyl-CoA hydrolysis Gallus gallus
2.3.3.10 0.038
-
 acetyl-CoA mutant H264N, acetyl-CoA hydrolysis Gallus gallus
2.3.3.10 0.058
-
 acetoacetyl-CoA mutant H264N Gallus gallus
2.3.3.10 0.115
-
 acetoacetyl-CoA mutant H264A Gallus gallus
2.3.3.10 0.116
-
 S-(3-oxobutyl)-CoA mutant H264A Gallus gallus
2.3.3.10 0.122
-
 acetyl-CoA mutant H264A Gallus gallus
2.3.3.10 0.172
-
 acetyl-CoA mutant H264N Gallus gallus
2.3.3.10 0.221
-
 acetyl-CoA mutant H436N Gallus gallus
2.3.3.10 0.294
-
 acetyl-CoA
-
 Gallus gallus
2.3.3.10 0.44
-
 acetyl-CoA mutant H197N Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 cytoplasm
-
 Gallus gallus 5737
-
2.3.3.10 cytoplasm
-
 Homo sapiens 5737
-
2.3.3.10 cytoplasm
-
 Rattus norvegicus 5737
-
2.3.3.10 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
2.3.3.10 cytoplasm
-
 Bos taurus 5737
-
2.3.3.10 mitochondrion
-
 Gallus gallus 5739
-
2.3.3.10 mitochondrion
-
 Mus musculus 5739
-
2.3.3.10 mitochondrion
-
 Homo sapiens 5739
-
2.3.3.10 mitochondrion
-
 Rattus norvegicus 5739
-
2.3.3.10 mitochondrion
-
 Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Mus musculus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Homo sapiens
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Bos taurus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Mesocricetus auratus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Blattella germanica
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Blattella germanica P54870 cockroach, cytosolic isoform 2
-
2.3.3.10 Blattella germanica P54961 cockroach, cytosolic isoform 1
-
2.3.3.10 Bos taurus
-
 ox
-
2.3.3.10 Gallus gallus
-
 avian
-
2.3.3.10 Homo sapiens
-
 human
-
2.3.3.10 Mesocricetus auratus
-
 syrian hamster, golden hamster
-
2.3.3.10 Mus musculus
-
 mouse
-
2.3.3.10 Rattus norvegicus
-
 rat
-
2.3.3.10 Saccharomyces cerevisiae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 liver
-
 Gallus gallus
-
2.3.3.10 liver
-
 Mus musculus
-
2.3.3.10 liver
-
 Rattus norvegicus
-
2.3.3.10 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.9 3-oxobutyl-CoA + acetyl-CoA
-
 Gallus gallus S-(3,5-dioxohexyl)-CoA
-
 ?
2.3.3.10 3-oxobutyryl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus 3-hydroxy-3-methyl-5-oxoheptanedioyl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mus musculus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Bos taurus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mesocricetus auratus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Blattella germanica (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 H2O + acetyl-CoA + S-(3-oxobutyl)-CoA
-
 Gallus gallus CoA + S-(4-carboxy-3-hydroxy-isoamyl)-CoA
-
 r