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Literature summary extracted from

  • Misra, I.; Narasimhan, C.; Miziorko, H.M.
    Avian 3-hydroxy-3-methylglutaryl-CoA synthase (1993), J. Biol. Chem., 268, 12129-12135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10 expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) Gallus gallus

General Stability

EC Number General Stability Organism
2.3.3.10 enzyme is vulnerable to proteolysis, expression in Escherichia coli leads to an quite stable enzyme Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 Mg2+ mitochondrial enzyme Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.012
-
 acetoacetyl-CoA recombinant enzyme, hydrolase reaction Gallus gallus
2.3.3.10 0.014
-
 acetyl-CoA wild-type, acetyl-CoA hydrolysis Gallus gallus
2.3.3.10 0.27
-
 acetyl-CoA reombinant enzyme Gallus gallus
2.3.3.10 0.3
-
 acetyl-CoA
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 cytosol
-
 Gallus gallus 5829
-
2.3.3.10 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.10 Mg2+ activity of the recombinant cytosolic isozyme ist stimulated by Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 57600
-
 SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Gallus gallus
-
 avian
-
2.3.3.10 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10 recombinant enzyme, expressed in Escherichia coli Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 liver
-
 Gallus gallus
-
2.3.3.10 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 0.5 1 liver enzyme Gallus gallus
2.3.3.10 0.8 1 recombinant enzyme Gallus gallus
2.3.3.10 1
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.3.3.10 -80°C, recombinant enzyme, negligible loss of activity after 1 year Gallus gallus
2.3.3.10 4°C, recombinant enzyme, loses 50% activity after 1 year Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + H2O in absence of co-substrate enzyme hydrolyzes acetyl-CoA Gallus gallus acetate + CoA
-
 ?