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Literature summary extracted from

  • Reed, W.D.; Lane, M.D.
    Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase from chicken liver (1975), Methods Enzymol., 35 B, 155-160.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.3.10 relatively stable dialyzed against 10 mM potassium phosphate in 0.1 mM dithiothreitol and EDTA for 4-10 days Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 acetoacetyl-CoA
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.05
-
 acetyl-CoA 0.005 mM acetoacetyl-CoA Gallus gallus
2.3.3.10 0.5
-
 acetyl-CoA 0.05 mM acetoacetyl-CoA Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 mitochondrion
-
 Gallus gallus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 53000
-
 2 * 53000, SDS-PAGE Gallus gallus
2.3.3.10 105000
-
 sedimentation equlibrium Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus potentially favorable position for the regulation of hepatic ketogenesis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Gallus gallus
-
 chicken
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 1
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.3.3.10 -20°C, may be stored in 30% glycerin solution for 2-3 months with no loss of activity Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O potentially favorable position for the regulation of hepatic ketogenesis Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.10 dimer 2 * 53000, SDS-PAGE Gallus gallus