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Literature summary extracted from

  • Clinkenbeard, K.D.; Sugiyama, T.; Lane, M.D.
    Cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase from chicken liver (1975), Methods Enzymol., 35 B, 160-167.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 5,5'-dithiobis(2-nitrobenzoate) Ellman's reagent Gallus gallus
2.3.3.10 5,5'-dithiobis(2-nitrobenzoate)
-
 Saccharomyces cerevisiae
2.3.3.10 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 acetoacetyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 p-chloromercuribenzoate
-
 Gallus gallus
2.3.3.10 p-chloromercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.3
-
 acetyl-CoA 0.1 mM acetoacetyl-CoA Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 cytoplasm
-
 Gallus gallus 5737
-
2.3.3.10 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
2.3.3.10 cytosol
-
 Gallus gallus 5829
-
2.3.3.10 cytosol
-
 Saccharomyces cerevisiae 5829
-
2.3.3.10 mitochondrion
-
 Gallus gallus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 55000
-
 2 * 55000, synthase II, SDS-PAGE Gallus gallus
2.3.3.10 100000
-
 cytosolic enzyme, sedimentation equilibrium Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus cytosolic synthase catalyzes the second step of cholesterogenesis from acetyl-CoA (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Gallus gallus
-
 chicken
-
2.3.3.10 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10 cytosolic synthase Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 0.3
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + 3'-dephospho-CoA + H2O
-
 Gallus gallus acetyldephospho-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O cytosolic synthase catalyzes the second step of cholesterogenesis from acetyl-CoA Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Subunits

EC Number Subunits Comment Organism
2.3.3.10 dimer 2 * 55000, synthase II, SDS-PAGE Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.10 9.4
-
-
 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.10 8 9.6
-
 Gallus gallus