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Literature summary extracted from

  • Brock, M.; Fischer, R.; Linder, D.; Buckel, W.
    Methylcitrate synthase from Aspergillus nidulans: implications for propionate as an antifungal agent (2000), Mol. Microbiol., 35, 961-973.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.5 gene mscA, DNA and amino acid sequence determination and analysis, overexpression in Aspergillus nidulans Aspergillus nidulans
2.3.3.16 cloning and expression in Aspergillus nidulans, DNA sequence Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.5 additional information construction of an enzyme deletion mutant strain, which is unable to grow on propionate, phenotype and growth behaviour, overview Aspergillus nidulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.5 ATP 30% inhibition at 3 mM Aspergillus nidulans
2.3.3.5 propionate
-
 Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.5 0.00063
-
 oxaloacetate pH 8.0, 25°C, purified native enzyme Aspergillus nidulans
2.3.3.5 0.0017
-
 propanoyl-CoA pH 8.0, 25°C, purified native enzyme Aspergillus nidulans
2.3.3.5 0.0025
-
 acetyl-CoA pH 8.0, 25°C, purified native enzyme Aspergillus nidulans
2.3.3.16 0.0017
-
 propionyl-CoA methylcitrate synthase exhibiting also citrate synthase activity Aspergillus nidulans
2.3.3.16 0.0025
-
 acetyl-CoA methylcitrate synthase exhibiting also citrate synthase activity Aspergillus nidulans
2.3.3.16 0.63
-
 oxaloacetate with propionyl-CoA, methylcitrate synthase exhibiting also citrate synthase activity Aspergillus nidulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.5 mitochondrial matrix
-
 Aspergillus nidulans 5759
-
2.3.3.5 mitochondrion enzyme possesses a mitochondrial signal import sequence Aspergillus nidulans 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.5 additional information Mg2+, Cu2+, Ni2+, Co2+, and EDTA do not have any effect on the enzyme Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.5 43000
-
 2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation Aspergillus nidulans
2.3.3.5 50580
-
 2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation Aspergillus nidulans
2.3.3.5 90000
-
 native enzyme, gel filtration Aspergillus nidulans
2.3.3.16 additional information
-
 amino acid sequence Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate Aspergillus nidulans reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate, key enzyme in the methylcitrate cycle for alpha-oxidation of propionate to pyruvate, overview (2S,3S)-2-methylcitrate + CoA
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.5 Aspergillus nidulans
-
 strains SMI45 and SMB/acuA, the latter is acetyl-CoA synthase-deficient, gene mscA
-
2.3.3.16 Aspergillus nidulans Q9TEM3 methylcitrate synthase, also exhibiting citrate synthase activity
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.3.5 proteolytic modification N-terminal processing of the mitochondrial signal import sequence Aspergillus nidulans

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.5 native enzyme 72.5fold from strain SMB/acuA Aspergillus nidulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.5 hypha
-
 Aspergillus nidulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.5 0.13
-
 strain SMI45 Aspergillus nidulans
2.3.3.5 0.2
-
 strain SMB/acuA Aspergillus nidulans
2.3.3.5 14.5
-
 purified native enzyme, with oxaloacetate and propionyl-CoA Aspergillus nidulans
2.3.3.5 41.5
-
 purified native enzyme, with oxaloacetate and acetyl-CoA Aspergillus nidulans
2.3.3.16 41.5
-
 citrate synthase activity of a purified methylcitrate synthase Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.5 acetyl-CoA + H2O + oxaloacetate the enzyme also performs the citrate synthase reaction, EC 2.3.3.1 Aspergillus nidulans citrate + CoA
-
 ir
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate, key enzyme in the methylcitrate cycle for alpha-oxidation of propionate to pyruvate, overview Aspergillus nidulans (2S,3S)-2-methylcitrate + CoA
-
 ir
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate Aspergillus nidulans (2S,3S)-2-methylcitrate + CoA
-
 ir
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Aspergillus nidulans citrate + CoA
-
 ?
2.3.3.16 propionyl-CoA + oxaloacetate + H2O methylcitrase synthase, also exhibiting citrate synthase activity Aspergillus nidulans 2-methylcitrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.5 dimer 2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
2.3.3.5 Methylcitrate synthase
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.5 45 52
-
 Aspergillus nidulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.5 20 70
-
 Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.5 23
-
 oxaloacetate pH 8.0, 25°C, purified native enzyme Aspergillus nidulans
2.3.3.5 23
-
 propanoyl-CoA pH 8.0, 25°C, purified native enzyme Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.5 8.5 9.5
-
 Aspergillus nidulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.5 5.5 10.5
-
 Aspergillus nidulans