Literature summary extracted from

Tabrett, C.A.; Copeland, L.
Biochemical controls of citrate synthase in chickpea bacteroids (2000), Arch. Microbiol., 173, 42-48.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.3.3.16	Urea	isocitrate, (R)-3-hydroxybutyrate, malonate, pyruvate, acetoacetyl have no effect	Mesorhizobium ciceri

EC Number	General Stability	Organism
2.3.3.16	freeze-drying results in 70% loss of activity	Mesorhizobium ciceri
2.3.3.16	KCl stabilizes	Mesorhizobium ciceri

EC Number	Inhibitors	Comment	Organism
2.3.3.16	2-oxoglutarate	30% inhibition at 1 mM	Cicer arietinum
2.3.3.16	2-oxoglutarate	no inhibition	Mesorhizobium ciceri
2.3.3.16	acetyl-CoA	no inhibition	Mesorhizobium ciceri
2.3.3.16	ADP	no inhibition	Mesorhizobium ciceri
2.3.3.16	AMP	no inhibition	Mesorhizobium ciceri
2.3.3.16	ATP	30% inhibition at 1 mM	Cicer arietinum
2.3.3.16	ATP	no inhibition	Mesorhizobium ciceri
2.3.3.16	CaCl2	50% inhibition at 10 mM, not reversible by KCl	Mesorhizobium ciceri
2.3.3.16	cAMP	no inhibition	Mesorhizobium ciceri
2.3.3.16	citrate	50% inhibition at 2.5 mM	Mesorhizobium ciceri
2.3.3.16	CoA	50% inhibition at 0.2 mM	Mesorhizobium ciceri
2.3.3.16	L-malate	no inhibition	Mesorhizobium ciceri
2.3.3.16	MgCl2	50% inhibition at 7 mM, not reversible by KCl	Mesorhizobium ciceri
2.3.3.16	additional information	isocitrate, (R)-3-hydroxybutyrate, malonate, pyruvate, acetoacetyl, NaCl, NH4Cl, CsCl, and RbCl have no effect	Mesorhizobium ciceri
2.3.3.16	NaCl	no effect	Mesorhizobium ciceri
2.3.3.16	NADH	no effect	Cicer arietinum
2.3.3.16	NADH	complete inhibition at 1 mM, completely reversible by NAD+	Mesorhizobium ciceri
2.3.3.16	NADP+	no inhibition	Mesorhizobium ciceri
2.3.3.16	NADPH	no inhibition	Mesorhizobium ciceri
2.3.3.16	oxaloacetate	no inhibition	Mesorhizobium ciceri

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.3.3.16	0.088	-	acetyl-CoA	in presence of 100 mM KCl	Mesorhizobium ciceri
2.3.3.16	0.174	-	acetyl-CoA	in absence of KCl	Mesorhizobium ciceri

EC Number	Metals/Ions	Comment	Organism	Structure
2.3.3.16	KCl	40% activation at 50 mM, 30% activation at 100 mM	Mesorhizobium ciceri
2.3.3.16	additional information	NaCl, NH4Cl, CsCl, and RbCl have no effect	Mesorhizobium ciceri

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.3.3.16	78000	-	gel filtration	Cicer arietinum
2.3.3.16	228000	-	gel filtration	Mesorhizobium ciceri

EC Number	Organism	UniProt	Comment	Textmining
2.3.3.16	Cicer arietinum	-	-	-
2.3.3.16	Mesorhizobium ciceri	-	bacteroids formed in symbiosis with Cicer arietinum plants	-
2.3.3.16	Mesorhizobium ciceri CC 1192	-	bacteroids formed in symbiosis with Cicer arietinum plants	-

EC Number	Purification (Comment)	Organism
2.3.3.16	partial	Cicer arietinum
2.3.3.16	partial	Mesorhizobium ciceri

EC Number	Source Tissue	Comment	Organism	Textmining
2.3.3.16	root nodule	-	Cicer arietinum	-
2.3.3.16	root nodule	bacteroids formed in symbiosis with Cicer arietinum plants	Mesorhizobium ciceri	-

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.3.3.16	0.87	-	partially purified enzyme	Cicer arietinum
2.3.3.16	1.25	-	partially purified enzyme	Mesorhizobium ciceri

EC Number	Storage Stability	Organism
2.3.3.16	4°C, 25 mM Tris-HCl, pH 7.7, loss of 80% activity within 3 days, 50 mM KCl protects	Mesorhizobium ciceri

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.3.3.16	acetyl-CoA + oxaloacetate + H2O	-	Cicer arietinum	citrate + CoA	-	?
2.3.3.16	acetyl-CoA + oxaloacetate + H2O	-	Mesorhizobium ciceri	citrate + CoA	-	?
2.3.3.16	acetyl-CoA + oxaloacetate + H2O	-	Mesorhizobium ciceri CC 1192	citrate + CoA	-	?

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.3.3.16	7.3	7.8	-	Mesorhizobium ciceri

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.3.3.16	0.023	-	NADH	-	Mesorhizobium ciceri