Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ruijter, G.J.G.; Panneman, H.; Xu, D.B.; Visser, J.
    Properties of Aspergillus niger citrate synthase and effects of citA overexpression on citric acid production (2000), FEMS Microbiol. Lett., 184, 35-40.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.16 cloning and expression of citA in Aspergillus niger, 11fold overexpression does not increase the citrate activity level in vivo Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.007
-
 oxaloacetate pH 7.5 Aspergillus niger
2.3.3.16 0.014
-
 acetyl-CoA pH 7.5 Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.16 mitochondrion N-terminal mitochondrial import signal sequence Aspergillus niger 5739
-
2.3.3.16 additional information N-terminal mitochondrial signal and C-terminal peroxisomal target sequence, unclear if both are functional Aspergillus niger
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 additional information
-
 C-terminal amino acid sequence alignment Aspergillus niger
2.3.3.16 additional information
-
 N-terminal protein sequence alignment Aspergillus niger
2.3.3.16 48000
-
 2 * 48000, mitochondrial enzyme, SDS-PAGE Aspergillus niger
2.3.3.16 80000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Aspergillus niger contributes little to flux control in the pathway citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 contributes little to flux control in the pathway citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Aspergillus niger Q9P8D6 gene citA
-
2.3.3.16 Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 Q9P8D6 gene citA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16
-
 Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.16 mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 79
-
 purified enzyme Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Aspergillus niger citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O contributes little to flux control in the pathway Aspergillus niger citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O contributes little to flux control in the pathway Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 dimer 2 * 48000, mitochondrial enzyme, SDS-PAGE Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 8.5
-
-
 Aspergillus niger