EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.16 | 2-oxoglutarate | no inhibition | Methylorubrum extorquens | |
2.3.3.16 | ADP | no inhibition | Methylorubrum extorquens | |
2.3.3.16 | ATP | - |
Methylorubrum extorquens | |
2.3.3.16 | NADH | no effect | Methylorubrum extorquens | |
2.3.3.16 | NADPH | no inhibition | Methylorubrum extorquens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.16 | 0.012 | - |
oxaloacetate | - |
Methylorubrum extorquens | |
2.3.3.16 | 0.084 | - |
acetyl-CoA | - |
Methylorubrum extorquens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.16 | KCl | up to 80 mM | Methylorubrum extorquens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | 66000 | - |
4 * 66000, SDS-PAGE | Methylorubrum extorquens |
2.3.3.16 | 260000 | - |
gel filtration | Methylorubrum extorquens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.16 | acetyl-CoA + oxaloacetate + H2O | Methylorubrum extorquens | involved in poly 3-hydroxybutyrate synthesis | citrate + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.16 | Methylorubrum extorquens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.16 | - |
Methylorubrum extorquens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | 24 | - |
purified enzyme | Methylorubrum extorquens |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.3.16 | 4°C, Tris-HCl buffer, pH8.0, 50 mM glycerol, at least 1 week | Methylorubrum extorquens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.16 | acetyl-CoA + oxaloacetate + H2O | - |
Methylorubrum extorquens | citrate + CoA | - |
? | |
2.3.3.16 | acetyl-CoA + oxaloacetate + H2O | involved in poly 3-hydroxybutyrate synthesis | Methylorubrum extorquens | citrate + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.3.16 | tetramer | 4 * 66000, SDS-PAGE | Methylorubrum extorquens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.16 | CS | - |
Methylorubrum extorquens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | 30 | - |
enzyme assay at | Methylorubrum extorquens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | 8.6 | 9 | - |
Methylorubrum extorquens |