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Literature summary extracted from

  • Lee, S.; Park, C.; Yim, J.
    Characterization of citrate synthase purified from Drosophila melanogaster (1997), Mol. Cells, 7, 599-604.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 2-oxoglutarate competitive against oxaloacetate Drosophila melanogaster
2.3.3.16 ATP competitive against acetyl-CoA Drosophila melanogaster
2.3.3.16 additional information tricarboxylic acid cycle intermediates Drosophila melanogaster
2.3.3.16 propionyl-CoA competitive against acetyl-CoA Drosophila melanogaster
2.3.3.16 succinyl-CoA mixed-type inhibition Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.0031
-
 oxaloacetate
-
 Drosophila melanogaster
2.3.3.16 0.0067
-
 acetyl-CoA
-
 Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 additional information
-
 amino acid composition Drosophila melanogaster
2.3.3.16 48700
-
 2 * 48700, SDS-PAGE Drosophila melanogaster
2.3.3.16 81000
-
 gel filtration Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 antibody studies Drosophila melanogaster

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.3.16 acetyl-CoA + H2O + oxaloacetate = citrate + CoA mechanism Drosophila melanogaster
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 112.5
-
 purified enzyme Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Drosophila melanogaster citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 dimer 2 * 48700, SDS-PAGE Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 45
-
-
 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 8 9
-
 Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 0.0024
-
 propionyl-CoA versus acetyl-CoA, competitive Drosophila melanogaster
2.3.3.16 0.0225
-
 propionyl-CoA versus oxaloacetate, noncompetitive Drosophila melanogaster
2.3.3.16 0.125
-
 ATP versus acetyl-CoA Drosophila melanogaster
2.3.3.16 0.38
-
 2-oxoglutarate versus oxaloacetate, competitive Drosophila melanogaster