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Literature summary extracted from

  • Sautter, C.; Keller, G.; Hock, B.
    Glyoxysomal citrate synthase from watermelon cotyledons: immunochemical localization and heterologous translation in Xenopus oocytes (1988), Planta, 173, 289-297.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.16 in vitro translation of glyoxysomal isozyme, heterologous expression in Xenopus laevis oocytes Citrullus lanatus subsp. vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 5,5'-dithiobis(2-nitrobenzoate) inactivation of the glyoxysomal isozyme, half-life: approx. 1 min, not mitochondrial isozyme Citrullus lanatus subsp. vulgaris
2.3.3.16 ATP
-
 Citrullus lanatus subsp. vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.16 glyoxysome
-
 Citrullus lanatus subsp. vulgaris 9514
-
2.3.3.16 mitochondrion
-
 Citrullus lanatus subsp. vulgaris 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 48000
-
 x * 48000, glyoxysomal isozyme, SDS-PAGE Citrullus lanatus subsp. vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Citrullus lanatus subsp. vulgaris key enzyme of glyoxylate cycle in fat-storing seedlings citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Citrullus lanatus subsp. vulgaris
-
 water melon, mitochondrial and glyoxysomal isoenzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 mitochondrial isozyme partially, glyoxysomal isozyme to homogeneity Citrullus lanatus subsp. vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.16 cotyledon
-
 Citrullus lanatus subsp. vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 0.764
-
 purified glyoxysomal isozyme Citrullus lanatus subsp. vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Citrullus lanatus subsp. vulgaris citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O key enzyme of glyoxylate cycle in fat-storing seedlings Citrullus lanatus subsp. vulgaris citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 ? x * 48000, glyoxysomal isozyme, SDS-PAGE Citrullus lanatus subsp. vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 25
-
 enzyme assay at Citrullus lanatus subsp. vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 8
-
 enzyme assay at Citrullus lanatus subsp. vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 0.33
-
 ATP mitochondrial isozyme Citrullus lanatus subsp. vulgaris
2.3.3.16 2.6
-
 ATP glyoxysomal isozyme Citrullus lanatus subsp. vulgaris