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Literature summary extracted from

  • Porter, J.S.; Wright, B.E.
    Partial purification and characterization of citrate synthase from Dictyostelium discoideum (1977), Arch. Biochem. Biophys., 181, 155-163.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.16 ammonium sulfate activates at 0.5 mM Dictyostelium discoideum

General Stability

EC Number General Stability Organism
2.3.3.16 destabilization by all salts, exception is ammonium sulfate, which stabilizes at low concentration Dictyostelium discoideum
2.3.3.16 dithiothreitol stabilizes for about 5 days Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 2-oxoglutarate no inhibition Dictyostelium discoideum
2.3.3.16 acetyl-CoA no inhibition Dictyostelium discoideum
2.3.3.16 ATP no inhibition Dictyostelium discoideum
2.3.3.16 CoA competitive with acetyl-CoA, noncompetitive with oxaloacetate Dictyostelium discoideum
2.3.3.16 NADH no effect Dictyostelium discoideum
2.3.3.16 oxaloacetate no inhibition Dictyostelium discoideum
2.3.3.16 p-chloromercuribenzoate can be restored by dithiothreitol treatment Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.007
-
 oxaloacetate
-
 Dictyostelium discoideum
2.3.3.16 0.01
-
 acetyl-CoA
-
 Dictyostelium discoideum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 (NH4)2SO4 activates at at least 0.5 mM Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 110000
-
 gel filtration Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Dictyostelium discoideum key enzyme of tricarboxylic acid cycle citrate + CoA
-
 r
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Dictyostelium discoideum NC-4 / ATCC 24697 key enzyme of tricarboxylic acid cycle citrate + CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Dictyostelium discoideum
-
-
-
2.3.3.16 Dictyostelium discoideum NC-4 / ATCC 24697
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 partial Dictyostelium discoideum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.3.16 acetyl-CoA + H2O + oxaloacetate = citrate + CoA ordered sequential mechanism Dictyostelium discoideum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 471.7
-
 partially purified enzyme Dictyostelium discoideum

Storage Stability

EC Number Storage Stability Organism
2.3.3.16 0°C, below, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol and 5 mg/ml bovine serum albumin, several months Dictyostelium discoideum
2.3.3.16 4°C, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol, 1 day Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Dictyostelium discoideum citrate + CoA
-
 r
2.3.3.16 acetyl-CoA + oxaloacetate + H2O key enzyme of tricarboxylic acid cycle Dictyostelium discoideum citrate + CoA
-
 r
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Dictyostelium discoideum NC-4 / ATCC 24697 citrate + CoA
-
 r
2.3.3.16 acetyl-CoA + oxaloacetate + H2O key enzyme of tricarboxylic acid cycle Dictyostelium discoideum NC-4 / ATCC 24697 citrate + CoA
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 23
-
 enzyme assay at Dictyostelium discoideum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.16 7.2 9
-
 Dictyostelium discoideum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 0.11
-
 CoA
-
 Dictyostelium discoideum