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Literature summary extracted from

  • Higa, A.; Cazzulo, J.J.
    Some properties of the citrate synthase from the extreme halophile, Halobacterium cutirubrum (1975), Biochem. J., 147, 267-274.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.3.3.16 dilute solutions instable, best stabilized by high concentrations of NaCl or KCl Halobacterium salinarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 additional information low salt concentrations inactivate reversibly Halobacterium salinarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 additional information
-
 additional information Km increases 5fold with salt concentration from 1 to 3 M, overview Halobacterium salinarum
2.3.3.16 0.018
-
 oxaloacetate minimum at 1 M KCl Halobacterium salinarum
2.3.3.16 0.26
-
 acetyl-CoA minimum at 1 M KCl Halobacterium salinarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 Ca2+ as CaCl2, inhibition at concentrations higher than 50 mM Halobacterium salinarum
2.3.3.16 K+ activation, Cl- most efficient anion Halobacterium salinarum
2.3.3.16 KCl maximal activation at 3 M Halobacterium salinarum
2.3.3.16 Mg2+ as MgCl2, inhibition at concentrations higher than 50 mM Halobacterium salinarum
2.3.3.16 NaCl
-
 Halobacterium salinarum

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Halobacterium salinarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16
-
 Halobacterium salinarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 additional information
-
-
 Halobacterium salinarum
2.3.3.16 27.45
-
-
 Halobacterium salinarum

Storage Stability

EC Number Storage Stability Organism
2.3.3.16 4°C, Tris-HCl buffer, pH 7.6, high protein concentration, below 20% loss of activity in 2 months Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Halobacterium salinarum citrate + CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 30
-
 enzyme assay at Halobacterium salinarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.16 additional information
-
 cold-lability in presence of 3 M KCl, but not with NaCl up to 5 M Halobacterium salinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 7.6
-
 assay at Halobacterium salinarum