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Literature summary extracted from

  • Johnson, D.E.; Hanson, R.S.
    Bacterial citrate synthases: purification, molecular weight and kinetic mechanism (1974), Biochim. Biophys. Acta, 350, 336-353.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.16 alpha-ketoglutarate
-
 Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 AMP slight activation Acinetobacter calcoaceticus subsp. anitratus

General Stability

EC Number General Stability Organism
2.3.3.16 very labile, glycerol, 20% v/v or more stabilizes Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 acetyl-CoA no inhibition Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 acetyl-CoA no inhibition Azotobacter vinelandii
2.3.3.16 acetyl-CoA substrate inhibition at high concentration Bacillus subtilis
2.3.3.16 ATP possibly involved in enzyme regulation Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 ATP possibly involved in enzyme regulation Azotobacter vinelandii
2.3.3.16 ATP possibly involved in enzyme regulation Bacillus subtilis
2.3.3.16 citrate competitive with acetyl-CoA, noncompetitive with oxaloacetate Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 citrate competitive with acetyl-CoA, noncompetitive with oxaloacetate Azotobacter vinelandii
2.3.3.16 citrate competitive with acetyl-CoA, noncompetitive with oxaloacetate Bacillus subtilis
2.3.3.16 CoA competitive with acetyl-CoA, noncompetitive with oxaloacetate Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 CoA competitive with acetyl-CoA, noncompetitive with oxaloacetate Azotobacter vinelandii
2.3.3.16 CoA competitive with acetyl-CoA, noncompetitive with oxaloacetate Bacillus subtilis
2.3.3.16 NADH 12% inhibition at 1 mM Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 NADH
-
 Azotobacter vinelandii
2.3.3.16 NADPH 29% inhibition at 0.25 mM Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 oxaloacetate no inhibition Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 oxaloacetate no inhibition Azotobacter vinelandii
2.3.3.16 oxaloacetate substrate inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.02
-
 oxaloacetate
-
 Azotobacter vinelandii
2.3.3.16 0.08
-
 acetyl-CoA
-
 Azotobacter vinelandii
2.3.3.16 0.1
-
 acetyl-CoA
-
 Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 0.6
-
 acetyl-CoA
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 KCl
-
 Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 KCl
-
 Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 58500
-
 4 * 58500, sedimentation equilibrium in guanidine-HCl and dithiothreitol Azotobacter vinelandii
2.3.3.16 60000 95000 gel filtration, sedimentation equilibrium centrifugation Bacillus subtilis
2.3.3.16 69000
-
 4 * 69000, sedimentation equilibrium in guanidine-HCl and dithiothreitol Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 242000
-
 sedimentation equilibrium centrifugation Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 250000
-
 sedimentation equilibrium centrifugation Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Bacillus subtilis entry step to tricarboxylic acid cycle citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Acinetobacter calcoaceticus subsp. anitratus entry step to tricarboxylic acid cycle citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Azotobacter vinelandii entry step to tricarboxylic acid cycle citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Bacillus subtilis HS2A2 entry step to tricarboxylic acid cycle citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Azotobacter vinelandii OP entry step to tricarboxylic acid cycle citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Acinetobacter calcoaceticus subsp. anitratus
-
-
-
2.3.3.16 Azotobacter vinelandii
-
-
-
2.3.3.16 Azotobacter vinelandii OP
-
-
-
2.3.3.16 Bacillus subtilis
-
-
-
2.3.3.16 Bacillus subtilis HS2A2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16
-
 Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16
-
 Azotobacter vinelandii
2.3.3.16 partial Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.3.16 acetyl-CoA + H2O + oxaloacetate = citrate + CoA random sequential reaction order mechanism Bacillus subtilis
a
2.3.3.16 acetyl-CoA + H2O + oxaloacetate = citrate + CoA random sequential reaction order mechanism Acinetobacter calcoaceticus subsp. anitratus
a
2.3.3.16 acetyl-CoA + H2O + oxaloacetate = citrate + CoA random sequential reaction order mechanism Azotobacter vinelandii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 2.6
-
 partially purified enzyme Bacillus subtilis
2.3.3.16 52.3
-
 purified enzyme Azotobacter vinelandii
2.3.3.16 235
-
 purified enzyme Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 523
-
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Bacillus subtilis citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Acinetobacter calcoaceticus subsp. anitratus citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Azotobacter vinelandii citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O entry step to tricarboxylic acid cycle Bacillus subtilis citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O entry step to tricarboxylic acid cycle Acinetobacter calcoaceticus subsp. anitratus citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O entry step to tricarboxylic acid cycle Azotobacter vinelandii citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Bacillus subtilis HS2A2 citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O entry step to tricarboxylic acid cycle Bacillus subtilis HS2A2 citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Azotobacter vinelandii OP citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O entry step to tricarboxylic acid cycle Azotobacter vinelandii OP citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 tetramer 4 * 58500, sedimentation equilibrium in guanidine-HCl and dithiothreitol Azotobacter vinelandii
2.3.3.16 tetramer 4 * 69000, sedimentation equilibrium in guanidine-HCl and dithiothreitol Acinetobacter calcoaceticus subsp. anitratus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 30
-
 enzyme assay at Bacillus subtilis
2.3.3.16 30
-
 enzyme assay at Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 30
-
 enzyme assay at Azotobacter vinelandii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.16 942
-
 citrate
-
 Acinetobacter calcoaceticus subsp. anitratus
2.3.3.16 2100
-
 citrate
-
 Azotobacter vinelandii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 0.75
-
 ATP versus acetyl-CoA Azotobacter vinelandii
2.3.3.16 1
-
 ATP
-
 Bacillus subtilis