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Literature summary extracted from

  • Danson, J.W.; Trawick, M.L.; Cooper, A.J.
    Spectrophotometric assays for L-lysine alpha-oxidase and gamma-glutamylamine cyclotransferase (2002), Anal. Biochem., 303, 120-130.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.14 Semicarbazide weak competitive, reversible inhibition Trichoderma viride

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.14 0.04
-
 L-lysine 70 mM potassium phosphate buffer pH 8.0 Trichoderma viride
1.4.3.14 0.4
-
 L-lysine 100 mM potassium phosphate buffer pH 7.4 and 500 mM semicarbazide Trichoderma viride

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.14 L-lysine + O2 + H2O Trichoderma viride
-
 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.14 Trichoderma viride
-
-
-
4.3.2.9 Bos taurus
-
-
-
4.3.2.9 Rattus norvegicus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.2.9 additional information
-
 spectrometric assay Rattus norvegicus
4.3.2.9 additional information
-
 spectrometric assay Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.14 L-leucine + O2 + H2O
-
 Trichoderma viride 4-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-lysine + O2 + H2O
-
 Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-lysine + semicarbazide + ?
-
 Trichoderma viride 4-methyl-2-oxopentanoate + ?
-
 ?
4.3.2.9 Nepsilon-(5-L-glutamyl)-L-lysine
-
 Rattus norvegicus 5-oxoproline + L-lysine
-
 ?
4.3.2.9 Nepsilon-(5-L-glutamyl)-L-lysine
-
 Bos taurus 5-oxoproline + L-lysine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.14 37
-
 assay at Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.14 7 8 Tris- and phosphate buffer Trichoderma viride
1.4.3.14 7.4
-
 assay at Trichoderma viride

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.14 FAD
-
 Trichoderma viride

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.14 56
-
 Semicarbazide 500 mM, 10 mM L-lysine, 3% inhibition Trichoderma viride