Literature summary extracted from

  • Orlowski, M.; Meister, A.
    gamma-Glutamyl cyclotransferase. Distribution, isozymic forms, and specificity (1973), J. Biol. Chem., 248, 2836-2844.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.9 100
-
alpha-N-(gamma-L-glutamyl)-L-lysine isoenzyme A Rattus norvegicus
4.3.2.9 0.6
-
5-L-glutamyl-5-L-glutamyl-p-nitroanilide
-
Rattus norvegicus
4.3.2.9 8
-
5-L-Glutamyl-L-alpha-aminobutyrate isoenzyme B Rattus norvegicus
4.3.2.9 10
-
5-L-Glutamyl-L-glutamine isoenzyme B Rattus norvegicus
4.3.2.9 12
-
5-L-Glutamyl-L-alpha-aminobutyrate isoenzyme A Rattus norvegicus
4.3.2.9 18
-
5-L-Glutamyl-L-glutamine isoenzyme A Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.3.2.9 cytoplasm
-
Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.9 27500
-
gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.9 Rattus norvegicus
-
Sprague-Dawley
-
4.3.2.9 Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.9 immunologically identical isoenzymes A and B, ammonium sulfate, gel filtration, carboxymethylcellulose, DEAE-cellulose Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.9 liver
-
Rattus norvegicus
-
4.3.2.9 kidney
-
Rattus norvegicus
-
4.3.2.9 brain
-
Rattus norvegicus
-
4.3.2.9 additional information
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.2.9 40
-
isoenzyme A, substrate 5-L-glutamyl-5-L-glutamyl-p-nitroanilide Rattus norvegicus
4.3.2.9 9.7
-
isoenzyme B, substrate 5-L-glutamyl-L-alpha-aminobutyrate Rattus norvegicus
4.3.2.9 8.9
-
isoenzyme B, substrate 5-L-glutamyl-5-L-glutamyl-p-nitroanilide Rattus norvegicus
4.3.2.9 38
-
isoenzyme A, substrate 5-L-glutamyl-L-alpha-aminobutyrate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.9 5-L-glutamyl-L-amino acid
-
Rattus norvegicus 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
4.3.2.9 5-L-glutamyl-L-amino acid
-
Rattus norvegicus Sprague-Dawley 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
4.3.2.9 5-L-glutamyl-L-methionine best substrate Rattus norvegicus 5-oxoproline + L-methionine
-
?
4.3.2.9 5-L-glutamyl-L-methionine best substrate Rattus norvegicus Sprague-Dawley 5-oxoproline + L-methionine
-
?
4.3.2.9 5-L-glutamyl-L-glutamine best substrate Rattus norvegicus 5-oxoproline + L-glutamine
-
?
4.3.2.9 5-L-glutamyl-L-glutamine best substrate Rattus norvegicus Sprague-Dawley 5-oxoproline + L-glutamine
-
?
4.3.2.9 5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
-
Rattus norvegicus 5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
-
?
4.3.2.9 5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
-
Rattus norvegicus Sprague-Dawley 5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
-
?
4.3.2.9 5-L-glutamyl-L-alpha-aminobutyrate
-
Rattus norvegicus 5-L-oxoproline + L-alpha-aminobutyrate
-
?
4.3.2.9 5-L-glutamyl-L-alpha-aminobutyrate
-
Rattus norvegicus Sprague-Dawley 5-L-oxoproline + L-alpha-aminobutyrate
-
?
4.3.2.9 5-L-glutamyl-glycine poor substrate Rattus norvegicus 5-oxoproline + glycine
-
?
4.3.2.9 5-L-glutamyl-L-6-N-benzyloxycarbonyl-L-lysine
-
Rattus norvegicus 5-oxoproline + L-6-N-benzyloxycarbonyl-L-lysine
-
?
4.3.2.9 5-L-glutamyl-L-glutamate poor substrate Rattus norvegicus 5-oxoproline + L-glutamate
-
?
4.3.2.9 5-L-glutamyl-L-leucine poor substrate Rattus norvegicus 5-oxoproline + L-leucine
-
?
4.3.2.9 2-N-(5-L-glutamyl)L-lysine
-
Rattus norvegicus 5-oxoproline + L-lysine
-
?
4.3.2.9 5-L-glutamyl-5-L-glutamyl-4-nitroanilide
-
Rattus norvegicus 5-oxoproline + 5-L-glutamyl-4-nitroanilide
-
?
4.3.2.9 alpha-N-(gamma-L-glutamyl)-L-lysine
-
Rattus norvegicus L-lysine + 5-oxo-L-proline
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.9 7.5 8
-
Rattus norvegicus