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Literature summary extracted from

  • Orlowski, M.; Richman, P.G.; Meister, A.
    Isolation and properties of gamma-L-glutamylcyclotransferase from human brain (1969), Biochemistry, 8, 1048-1055.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.9 0.4
-
 5-L-Glutamyl-5-L-glutamyl-4-nitroanilide
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.3.2.9 cytoplasm
-
 Homo sapiens 5737
-
4.3.2.9 cytoplasm
-
 Ovis aries 5737
-

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.9 Homo sapiens
-
-
-
4.3.2.9 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.9
-
 Ovis aries
4.3.2.9 pH 4.2, ammonium sulfate, heat, Sephadex G-75, carboxymethyl cellulose, DEAE-cellulose Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.9 brain
-
 Homo sapiens
-
4.3.2.9 brain
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.2.9 100
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
4.3.2.9 -20°C, stable Homo sapiens
4.3.2.9 -20°C, stable Ovis aries
4.3.2.9 0°C, at least 1 month Homo sapiens
4.3.2.9 0°C, at least 1 month Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.9 5-L-glutamyl-5-L-glutamyl-4-nitroanilide
-
 Homo sapiens 5-oxoproline + 5-L-glutamyl-4-nitroanilide
-
 ?
4.3.2.9 5-L-glutamyl-glycine
-
 Homo sapiens 5-oxoproline + glycine
-
 ?
4.3.2.9 5-L-glutamyl-glycine
-
 Ovis aries 5-oxoproline + glycine
-
 ?
4.3.2.9 5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide best substrate Homo sapiens 5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
-
 ?
4.3.2.9 5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide best substrate Ovis aries 5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
-
 ?
4.3.2.9 5-L-glutamyl-L-alanine
-
 Homo sapiens 5-L-oxoproline + L-alanine
-
 ?
4.3.2.9 5-L-glutamyl-L-alanine
-
 Ovis aries 5-L-oxoproline + L-alanine
-
 ?
4.3.2.9 5-L-glutamyl-L-alpha-aminobutyrate
-
 Homo sapiens 5-L-oxoproline + L-alpha-aminobutyrate
-
 ?
4.3.2.9 5-L-glutamyl-L-alpha-aminobutyrate
-
 Ovis aries 5-L-oxoproline + L-alpha-aminobutyrate
-
 ?
4.3.2.9 5-L-glutamyl-L-amino acid no activity with LD-, DD- and DL-isomers of 5-L-glutamyl-5-L-glutamyl-4-nitroanilide Homo sapiens 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
4.3.2.9 5-L-glutamyl-L-amino acid no activity with LD-, DD- and DL-isomers of 5-L-glutamyl-5-L-glutamyl-4-nitroanilide Ovis aries 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
4.3.2.9 5-L-glutamyl-L-amino acid no activity with: glutathione, i.e. gamma-L-glutamyl-L-cysteinylglycine Homo sapiens 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
4.3.2.9 5-L-glutamyl-L-amino acid no activity with: glutathione, i.e. gamma-L-glutamyl-L-cysteinylglycine Ovis aries 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
4.3.2.9 5-L-glutamyl-L-amino acid no activity with: 5-glutamyl-4-nitroanilide Homo sapiens 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
4.3.2.9 5-L-glutamyl-L-amino acid no activity with: 5-glutamyl-4-nitroanilide Ovis aries 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
4.3.2.9 5-L-glutamyl-L-glutamine
-
 Homo sapiens 5-oxoproline + L-glutamine
-
 ?
4.3.2.9 5-L-glutamyl-L-glutamine
-
 Ovis aries 5-oxoproline + L-glutamine
-
 ?
4.3.2.9 5-L-glutamyl-S-methyl-L-cysteine
-
 Homo sapiens 5-oxoproline + S-methyl-L-cysteine
-
 ?
4.3.2.9 5-L-glutamyl-S-methyl-L-cysteine
-
 Ovis aries 5-oxoproline + S-methyl-L-cysteine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.2.9 57
-
 purified enzyme, complete loss of activity after 5 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.9 7.8 8.2
-
 Homo sapiens
4.3.2.9 7.8 8.2
-
 Ovis aries