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Literature summary extracted from

  • Hussein, A.S.; Walter, R.D.
    Purification and characterization of gamma-glutamyl transpeptidase from Ascaris suum (1996), Mol. Biochem. Parasitol., 77, 41-47.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 acivicin
-
 Ascaris suum
2.3.2.2 D-serine
-
 Ascaris suum
2.3.2.2 L- or D-Serine/borate competitive Ascaris suum
2.3.2.2 L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazolacetic acid i.e. acivicin; in vivo inhibition and accumulation of glutathione; kinetics; strong, irreversible, reacts with glutamyl binding site Ascaris suum
2.3.2.2 L-serine
-
 Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 additional information
-
 additional information kinetic study Ascaris suum
2.3.2.2 additional information
-
 additional information Km values for several amino acids and dipeptides, overview Ascaris suum
2.3.2.2 0.03
-
 5-L-glutamyl-7-amido-4-methylcoumarin
-
 Ascaris suum
2.3.2.2 1.28
-
 glycylglycine
-
 Ascaris suum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 membrane
-
 Ascaris suum 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 30000
-
 1 * 43000 + 1 * 30000, SDS-PAGE Ascaris suum
2.3.2.2 43000
-
 1 * 43000 + 1 * 30000, SDS-PAGE Ascaris suum
2.3.2.2 70000
-
 gel filtration Ascaris suum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Ascaris suum
-
 peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 glutathione + an amino acid Ascaris suum initial step of glutathione degradation L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Ascaris suum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 solubilized by Triton X-100 Ascaris suum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid kinetic study Ascaris suum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 additional information
-
 activities with diverse substrates Ascaris suum
2.3.2.2 1009
-
 purified enzyme Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Ascaris suum peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Ascaris suum peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-7-amido-4-methylcoumarin + acceptor acceptors are amino acids and peptides, overview Ascaris suum 7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
-
 ?
2.3.2.2 5-L-glutamyl-7-amido-4-methylcoumarin + acceptor glycylglycine is best acceptor Ascaris suum 7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
-
 ?
2.3.2.2 glutathione + an amino acid initial step of glutathione degradation Ascaris suum L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
 ?
2.3.2.2 L-glutamyl-gamma-7-amido-4-methylcoumarin + glycylglycine
-
 Ascaris suum 7-amino-4-methylcoumarin + L-glutamyl-glycylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 43000 + 1 * 30000, SDS-PAGE Ascaris suum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Ascaris suum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 8.5
-
 assay at Ascaris suum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.2 0.3
-
 L-serine in presence of borate Ascaris suum
2.3.2.2 0.42
-
 acivicin
-
 Ascaris suum
2.3.2.2 0.61
-
 D-serine in presence of borate Ascaris suum