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Literature summary extracted from

  • Huseby, N.
    Purification and some properties of gamma-glutamyltransferase from human liver (1977), Biochim. Biophys. Acta, 483, 46-56.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.81
-
 L-Glu-4-nitroanilide
-
 Homo sapiens
2.3.2.2 12.4
-
 glycylglycine
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 22000
-
 1 * 47000 + 1 * 22000, SDS-PAGE Homo sapiens
2.3.2.2 47000
-
 1 * 47000 + 1 * 22000, SDS-PAGE Homo sapiens
2.3.2.2 90000
-
-
 Homo sapiens
2.3.2.2 110000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein binding of concanavalin A Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 423
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 47000 + 1 * 22000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 7.6
-
 assay at Homo sapiens