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Literature summary extracted from

  • Tate, S.S.; Ross, M.E.
    Human kidney gamma-glutamyl transpeptidase. Catalytic properties, subunit structure, and localization of the gamma-glutamyl binding site on the light subunit (1977), J. Biol. Chem., 252, 6042-6045.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 Maleate
-
 Rattus norvegicus
2.3.2.2 Maleate stimulates hydrolase reaction 3fold, inhibits transpeptidase reaction, with L-Glu as substrate Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 6-diazo-5-oxo-L-norleucine 5-glutamyl-donor, protect transpeptidase and hydrolase activity, not 5-carbon derivative; i.e. DON, strong, irreversible modification of glutamyl-binding site of light subunit Homo sapiens
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
 Rattus norvegicus
2.3.2.2 L-azaserine irreversible inactivation; not 5-carbon derivative; transpeptidase and hydrolase reaction, glutamyl-donor protects Homo sapiens
2.3.2.2 L-azaserine irreversible inactivation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 additional information
-
 amino acid composition Homo sapiens
2.3.2.2 additional information
-
 amino acid composition Rattus norvegicus
2.3.2.2 22000
-
 1 * 62000 + 1 * 22000, SDS-PAGE Homo sapiens
2.3.2.2 62000
-
 1 * 62000 + 1 * 22000, SDS-PAGE Homo sapiens
2.3.2.2 86000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Homo sapiens
-
-
-
2.3.2.2 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein
-
 Homo sapiens
2.3.2.2 glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 kidney
-
 Homo sapiens
-
2.3.2.2 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 800
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is 5-L-Glu-4-nitroanilide Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is 5-L-Glu-4-nitroanilide Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: S-acetophenoneglutathione Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: S-acetophenoneglutathione Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: S-acetylglutathione Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-alanine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-alanine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-asparagine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-methionine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-methionine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-serine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-L-serine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-serine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-serine
-
 ?
2.3.2.2 glutathione + acceptor
-
 Homo sapiens cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
2.3.2.2 glutathione + acceptor
-
 Rattus norvegicus cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer
-
 Rattus norvegicus
2.3.2.2 dimer 1 * 62000 + 1 * 22000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Homo sapiens
2.3.2.2 37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.2 1150
-
 4-nitroanilide
-
 Homo sapiens
2.3.2.2 1250
-
 4-nitroanilide
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 additional information
-
-
 Rattus norvegicus
2.3.2.2 additional information
-
 several isozymic forms with different content of sialic acid Homo sapiens