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Literature summary extracted from

  • Miller, S.P.; Awasthi, Y.C.; Srivastava, S.K.
    Studies of human kidney gamma-glutamyl transpeptidase. Purification and structural, kinetic and immunological properties (1976), J. Biol. Chem., 251, 2271-2278.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 additional information effect of amino acids on activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 Co2+ weak Homo sapiens
2.3.2.2 glutathione
-
 Homo sapiens
2.3.2.2 iodoacetate weak Homo sapiens
2.3.2.2 Maleate
-
 Homo sapiens
2.3.2.2 additional information inhibition by diverse amino acids, overview Homo sapiens
2.3.2.2 N-ethylmaleimide
-
 Homo sapiens
2.3.2.2 NH4+ weak Homo sapiens
2.3.2.2 p-hydroxymercuribenzoate weak Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.8
-
 L-Glu-4-nitroanilide
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.2 Ca2+ no effect Homo sapiens
2.3.2.2 K+ no effect Homo sapiens
2.3.2.2 Li+ no effect Homo sapiens
2.3.2.2 Mg2+ no effect Homo sapiens
2.3.2.2 additional information no activation by Zn2+ and Mn2+ Homo sapiens
2.3.2.2 Na+ no effect Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 additional information
-
 amino acid composition Homo sapiens
2.3.2.2 additional information
-
 in the presence of Triton X-100: molecular aggregation Homo sapiens
2.3.2.2 21000
-
 1 * 57000 + 1 * 21000, SDS-PAGE, presence of urea, 2-mercaptoethanol Homo sapiens
2.3.2.2 57000
-
 1 * 57000 + 1 * 21000, SDS-PAGE, presence of urea, 2-mercaptoethanol Homo sapiens
2.3.2.2 90000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 kidney
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 200.4
-
 purified enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.3.2.2 4°C, t1/2: 2 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
2.3.2.2 glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Homo sapiens L-cysteinylglycine + L-glutamate
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 57000 + 1 * 21000, SDS-PAGE, presence of urea, 2-mercaptoethanol Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 additional information
-
 pI: 4.5 Homo sapiens
2.3.2.2 8
-
 assay at Homo sapiens
2.3.2.2 8.2
-
-
 Homo sapiens