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Literature summary extracted from

  • Takahashi, S.; Zukin, R.S.; Steinman, H.M.
    gamma-Glutamyl transpeptidase from WI-38 fibroblasts: purification and active site modification studies (1981), Arch. Biochem. Biophys., 207, 87-95.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 5-Iodoacetamidofluorescein inactivation, active site modification Homo sapiens
2.3.2.2 6-diazo-5-oxo-L-norleucine complete inactivation, accelerated by maleate, prevented by S-methylglutathione; i.e. DON, strong, irreversible modification of glutamyl-binding site of light subunit; maleate accelerates inactivation; S-methylglutathione Homo sapiens
2.3.2.2 L-azaserine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 1
-
 L-Glu-4-nitroanilide
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 additional information
-
 amino acid composition Homo sapiens
2.3.2.2 additional information
-
 in the presence of Triton X-100: molecular aggregation Homo sapiens
2.3.2.2 additional information
-
 protease solubilized enzymes have a reduced MW Homo sapiens
2.3.2.2 20000
-
 1 * 53000 + 1 * 20000, papain-solubilized, SDS-PAGE Homo sapiens
2.3.2.2 20000
-
 1 * 62000 + 1 * 20000, SDS-PAGE Homo sapiens
2.3.2.2 53000
-
 1 * 53000 + 1 * 20000, papain-solubilized, SDS-PAGE Homo sapiens
2.3.2.2 62000
-
 1 * 62000 + 1 * 20000, SDS-PAGE Homo sapiens
2.3.2.2 71000
-
 papain-solubilized, gel filtration Homo sapiens
2.3.2.2 82000
-
 papain-solubilized enzyme, gel filtration Homo sapiens
2.3.2.2 160000
-
 Triton X-100 solubilized, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 cell culture
-
 Homo sapiens
-
2.3.2.2 lung
-
 Homo sapiens
-
2.3.2.2 lung fibroblast WI-38 fetal Homo sapiens
-
2.3.2.2 WI-38 cell whole body, fetal Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 16
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 53000 + 1 * 20000, papain-solubilized, SDS-PAGE Homo sapiens
2.3.2.2 dimer 1 * 62000 + 1 * 20000, SDS-PAGE Homo sapiens
2.3.2.2 More protease solubilization leads to a reduced MW of the larger subunit Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 8
-
-
 Homo sapiens