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Literature summary extracted from

  • Tate, S.S.; Meister, A.
    gamma-Glutamyl transpeptidase from kidney (1985), Methods Enzymol., 113, 400-419.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 Free bile acids activation Mammalia
2.3.2.2 Free bile acids activation Mus musculus
2.3.2.2 Free bile acids activation Rattus norvegicus
2.3.2.2 Free bile acids and their glycine and taurine conjugates Mammalia
2.3.2.2 Free bile acids and their glycine and taurine conjugates Mus musculus
2.3.2.2 Free bile acids and their glycine and taurine conjugates Rattus norvegicus
2.3.2.2 hippurate stimulates hydrolase reaction, inhibits transpeptidation Mammalia
2.3.2.2 hippurate stimulates hydrolase reaction, inhibits transpeptidation Mus musculus
2.3.2.2 hippurate stimulates hydrolase reaction, inhibits transpeptidation Rattus norvegicus
2.3.2.2 Maleate
-
 Mammalia
2.3.2.2 Maleate
-
 Mus musculus
2.3.2.2 Maleate
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 5-glutamylhydrazones
-
 Mammalia
2.3.2.2 5-glutamylhydrazones
-
 Mus musculus
2.3.2.2 5-glutamylhydrazones
-
 Rattus norvegicus
2.3.2.2 5-glutamylphenylhydrazides
-
 Mammalia
2.3.2.2 5-glutamylphenylhydrazides
-
 Mus musculus
2.3.2.2 5-glutamylphenylhydrazides
-
 Rattus norvegicus
2.3.2.2 5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective Mammalia
2.3.2.2 5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective Mus musculus
2.3.2.2 5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective Rattus norvegicus
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
 Mammalia
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
 Mus musculus
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
 Rattus norvegicus
2.3.2.2 Acetazolamide Maleate transpeptidase, not hydrolase reaction Mammalia
2.3.2.2 Acetazolamide Maleate transpeptidase, not hydrolase reaction Mus musculus
2.3.2.2 Acetazolamide Maleate transpeptidase, not hydrolase reaction Rattus norvegicus
2.3.2.2 Free bile acids and their glycine and taurine conjugates
-
 Mammalia
2.3.2.2 Free bile acids and their glycine and taurine conjugates
-
 Mus musculus
2.3.2.2 Free bile acids and their glycine and taurine conjugates
-
 Rattus norvegicus
2.3.2.2 hippurate transpeptidase, not hydrolase reaction Mammalia
2.3.2.2 hippurate transpeptidase, not hydrolase reaction Mus musculus
2.3.2.2 hippurate transpeptidase, not hydrolase reaction Rattus norvegicus
2.3.2.2 L- or D-Serine/borate
-
 Mammalia
2.3.2.2 L- or D-Serine/borate
-
 Mus musculus
2.3.2.2 L- or D-Serine/borate
-
 Rattus norvegicus
2.3.2.2 L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid i.e. AT-125; strong, irreversible Mammalia
2.3.2.2 L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid i.e. AT-125; strong, irreversible Mus musculus
2.3.2.2 L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid i.e. AT-125; strong, irreversible Rattus norvegicus
2.3.2.2 L-azaserine irreversible inactivation Mammalia
2.3.2.2 L-azaserine irreversible inactivation Mus musculus
2.3.2.2 L-azaserine irreversible inactivation Rattus norvegicus
2.3.2.2 additional information
-
 Mammalia
2.3.2.2 additional information
-
 Mus musculus
2.3.2.2 additional information
-
 Rattus norvegicus
2.3.2.2 PMSF
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 additional information
-
 additional information
-
 Mammalia
2.3.2.2 additional information
-
 additional information
-
 Mus musculus
2.3.2.2 additional information
-
 additional information
-
 Rattus norvegicus
2.3.2.2 0.005 0.0068 L-Glu-4-nitroanilide hydrolase reaction Rattus norvegicus
2.3.2.2 0.029 0.035 L-cystine hydrolase reaction Rattus norvegicus
2.3.2.2 0.029 0.035 L-cystine D-Glu-4-nitroanilide Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 brush border jejunum, microvilli Mammalia 5903
-
2.3.2.2 brush border jejunum, microvilli Mus musculus 5903
-
2.3.2.2 brush border jejunum, microvilli Rattus norvegicus 5903
-
2.3.2.2 extracellular
-
 Mammalia
-
-
2.3.2.2 extracellular
-
 Mus musculus
-
-
2.3.2.2 extracellular
-
 Rattus norvegicus
-
-
2.3.2.2 membrane intrinsic membrane protein Mammalia 16020
-
2.3.2.2 membrane intrinsic membrane protein Mus musculus 16020
-
2.3.2.2 membrane intrinsic membrane protein Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 22000
-
 1 * 51000 + 1 * 22000, Triton X-100 solubilized, SDS-PAGE Rattus norvegicus
2.3.2.2 22000
-
 1 * 46000 + 1 * 22000, papain solubilized, SDS-PAGE Rattus norvegicus
2.3.2.2 46000
-
 1 * 46000 + 1 * 22000, papain solubilized, SDS-PAGE Rattus norvegicus
2.3.2.2 51000
-
 1 * 51000 + 1 * 22000, Triton X-100 solubilized, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Mammalia involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 r
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Mus musculus involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 r
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Rattus norvegicus involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 r
2.3.2.2 glutathione + an amino acid Mammalia involved in 5-L-glutamyl cycle of glutathione metabolism L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid Mus musculus involved in 5-L-glutamyl cycle of glutathione metabolism L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid Rattus norvegicus involved in 5-L-glutamyl cycle of glutathione metabolism L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid Mammalia initial step of glutathione degradation L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid Mus musculus initial step of glutathione degradation L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid Rattus norvegicus initial step of glutathione degradation L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Mammalia
-
-
-
2.3.2.2 Mus musculus
-
-
-
2.3.2.2 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein
-
 Mammalia
2.3.2.2 glycoprotein both subunits are glycoproteins, structure Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 solubilized with either detergents or proteinases Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Mammalia
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Mus musculus
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Rattus norvegicus
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid structure Mammalia
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid structure Mus musculus
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid structure Rattus norvegicus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.2.2
-
 Mammalia
2.3.2.2
-
 Mus musculus
2.3.2.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 epididymis
-
 Mammalia
-
2.3.2.2 epididymis
-
 Mus musculus
-
2.3.2.2 epididymis
-
 Rattus norvegicus
-
2.3.2.2 intestine
-
 Mammalia
-
2.3.2.2 intestine
-
 Mus musculus
-
2.3.2.2 intestine
-
 Rattus norvegicus
-
2.3.2.2 jejunum outer space of epithelial membranes Mammalia
-
2.3.2.2 jejunum outer space of epithelial membranes Mus musculus
-
2.3.2.2 jejunum outer space of epithelial membranes Rattus norvegicus
-
2.3.2.2 kidney
-
 Mammalia
-
2.3.2.2 kidney
-
 Mus musculus
-
2.3.2.2 kidney
-
 Rattus norvegicus
-
2.3.2.2 liver
-
 Mammalia
-
2.3.2.2 liver
-
 Mus musculus
-
2.3.2.2 liver
-
 Rattus norvegicus
-
2.3.2.2 additional information distribution Mammalia
-
2.3.2.2 additional information distribution Mus musculus
-
2.3.2.2 additional information distribution Rattus norvegicus
-
2.3.2.2 pancreas
-
 Mammalia
-
2.3.2.2 pancreas
-
 Mus musculus
-
2.3.2.2 pancreas
-
 Rattus norvegicus
-
2.3.2.2 seminal plasma
-
 Mammalia
-
2.3.2.2 seminal plasma
-
 Mus musculus
-
2.3.2.2 seminal plasma
-
 Rattus norvegicus
-
2.3.2.2 spleen
-
 Mammalia
-
2.3.2.2 spleen
-
 Mus musculus
-
2.3.2.2 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 738
-
 purified Triton X-100 solubilized kidney enzyme Rattus norvegicus
2.3.2.2 810
-
 purified papain solubilized kidney enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor binding site reacts with L- and to a lesser extent D-enantiomer Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor binding site reacts with L- and to a lesser extent D-enantiomer Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor binding site reacts with L- and to a lesser extent D-enantiomer Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ strict L-stereospecificity of 5-Glu-acceptor site Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ strict L-stereospecificity of 5-Glu-acceptor site Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ strict L-stereospecificity of 5-Glu-acceptor site Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Mammalia peptide + 5-L-glutamyl amino acid
-
 r
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Mus musculus peptide + 5-L-glutamyl amino acid
-
 r
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 r
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Mammalia 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Mus musculus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-cystine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-cystine
-
 ?
2.3.2.2 5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
 Mammalia 7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
 Mus musculus 7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
 Rattus norvegicus 7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
 ?
2.3.2.2 glutathione + acceptor acceptor: amino acids Mammalia cysteinylglycine + 5-L-glutamyl-acceptor
-
 r
2.3.2.2 glutathione + acceptor acceptor: amino acids Mus musculus cysteinylglycine + 5-L-glutamyl-acceptor
-
 r
2.3.2.2 glutathione + acceptor acceptor: amino acids Rattus norvegicus cysteinylglycine + 5-L-glutamyl-acceptor
-
 r
2.3.2.2 glutathione + an amino acid involved in 5-L-glutamyl cycle of glutathione metabolism Mammalia L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid involved in 5-L-glutamyl cycle of glutathione metabolism Mus musculus L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid involved in 5-L-glutamyl cycle of glutathione metabolism Rattus norvegicus L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid initial step of glutathione degradation Mammalia L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid initial step of glutathione degradation Mus musculus L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + an amino acid initial step of glutathione degradation Rattus norvegicus L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ir
2.3.2.2 glutathione + glutathione autotranspeptidation Mammalia L-cysteinylglycine + 5-glutamyl-glutathione
-
 r
2.3.2.2 glutathione + glutathione autotranspeptidation Mus musculus L-cysteinylglycine + 5-glutamyl-glutathione
-
 r
2.3.2.2 glutathione + glutathione autotranspeptidation Rattus norvegicus L-cysteinylglycine + 5-glutamyl-glutathione
-
 r
2.3.2.2 glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Mammalia L-cysteinylglycine + L-glutamate
-
 ir
2.3.2.2 glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Mus musculus L-cysteinylglycine + L-glutamate
-
 ir
2.3.2.2 glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Rattus norvegicus L-cysteinylglycine + L-glutamate
-
 ir
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 51000 + 1 * 22000, Triton X-100 solubilized, SDS-PAGE Rattus norvegicus
2.3.2.2 dimer 1 * 46000 + 1 * 22000, papain solubilized, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 additional information
-
 12 isozymes with pI-values ranging from 5 to 8 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.2 additional information
-
 additional information Ki values for diverse amino acids, overview Mammalia
2.3.2.2 additional information
-
 additional information Ki values for diverse amino acids, overview Mus musculus
2.3.2.2 additional information
-
 additional information Ki values for diverse amino acids, overview Rattus norvegicus