BRENDA - Enzyme Database

gamma-Glutamyl transpeptidase from kidney

Tate, S.S.; Meister, A.; Methods Enzymol. 113, 400-419 (1985) View publication on PubMed

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.2.2
Free bile acids
activation
Mammalia
2.3.2.2
Free bile acids
activation
Mus musculus
2.3.2.2
Free bile acids
activation
Rattus norvegicus
2.3.2.2
Free bile acids
and their glycine and taurine conjugates
Mammalia
2.3.2.2
Free bile acids
and their glycine and taurine conjugates
Mus musculus
2.3.2.2
Free bile acids
and their glycine and taurine conjugates
Rattus norvegicus
2.3.2.2
hippurate
stimulates hydrolase reaction, inhibits transpeptidation
Mammalia
2.3.2.2
hippurate
stimulates hydrolase reaction, inhibits transpeptidation
Mus musculus
2.3.2.2
hippurate
stimulates hydrolase reaction, inhibits transpeptidation
Rattus norvegicus
2.3.2.2
Maleate
-
Mammalia
2.3.2.2
Maleate
-
Mus musculus
2.3.2.2
Maleate
-
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.2.2
5-glutamylhydrazones
-
Mammalia
2.3.2.2
5-glutamylhydrazones
-
Mus musculus
2.3.2.2
5-glutamylhydrazones
-
Rattus norvegicus
2.3.2.2
5-glutamylphenylhydrazides
-
Mammalia
2.3.2.2
5-glutamylphenylhydrazides
-
Mus musculus
2.3.2.2
5-glutamylphenylhydrazides
-
Rattus norvegicus
2.3.2.2
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective
Mammalia
2.3.2.2
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective
Mus musculus
2.3.2.2
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective
Rattus norvegicus
2.3.2.2
6-diazo-5-oxo-L-norleucine
-
Mammalia
2.3.2.2
6-diazo-5-oxo-L-norleucine
-
Mus musculus
2.3.2.2
6-diazo-5-oxo-L-norleucine
-
Rattus norvegicus
2.3.2.2
Acetazolamide Maleate
transpeptidase, not hydrolase reaction
Mammalia
2.3.2.2
Acetazolamide Maleate
transpeptidase, not hydrolase reaction
Mus musculus
2.3.2.2
Acetazolamide Maleate
transpeptidase, not hydrolase reaction
Rattus norvegicus
2.3.2.2
Free bile acids and their glycine and taurine conjugates
-
Mammalia
2.3.2.2
Free bile acids and their glycine and taurine conjugates
-
Mus musculus
2.3.2.2
Free bile acids and their glycine and taurine conjugates
-
Rattus norvegicus
2.3.2.2
hippurate
transpeptidase, not hydrolase reaction
Mammalia
2.3.2.2
hippurate
transpeptidase, not hydrolase reaction
Mus musculus
2.3.2.2
hippurate
transpeptidase, not hydrolase reaction
Rattus norvegicus
2.3.2.2
L- or D-Serine/borate
-
Mammalia
2.3.2.2
L- or D-Serine/borate
-
Mus musculus
2.3.2.2
L- or D-Serine/borate
-
Rattus norvegicus
2.3.2.2
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
i.e. AT-125; strong, irreversible
Mammalia
2.3.2.2
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
i.e. AT-125; strong, irreversible
Mus musculus
2.3.2.2
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
i.e. AT-125; strong, irreversible
Rattus norvegicus
2.3.2.2
L-azaserine
irreversible inactivation
Mammalia
2.3.2.2
L-azaserine
irreversible inactivation
Mus musculus
2.3.2.2
L-azaserine
irreversible inactivation
Rattus norvegicus
2.3.2.2
additional information
-
Mammalia
2.3.2.2
additional information
-
Mus musculus
2.3.2.2
additional information
-
Rattus norvegicus
2.3.2.2
PMSF
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.2.2
additional information
-
additional information
-
Mammalia
2.3.2.2
additional information
-
additional information
-
Mus musculus
2.3.2.2
additional information
-
additional information
-
Rattus norvegicus
2.3.2.2
0.005
0.0068
L-Glu-4-nitroanilide
hydrolase reaction
Rattus norvegicus
2.3.2.2
0.029
0.035
L-cystine
hydrolase reaction
Rattus norvegicus
2.3.2.2
0.029
0.035
L-cystine
D-Glu-4-nitroanilide
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.2.2
brush border
jejunum, microvilli
Mammalia
5903
-
2.3.2.2
brush border
jejunum, microvilli
Mus musculus
5903
-
2.3.2.2
brush border
jejunum, microvilli
Rattus norvegicus
5903
-
2.3.2.2
extracellular
-
Mammalia
-
-
2.3.2.2
extracellular
-
Mus musculus
-
-
2.3.2.2
extracellular
-
Rattus norvegicus
-
-
2.3.2.2
membrane
intrinsic membrane protein
Mammalia
16020
-
2.3.2.2
membrane
intrinsic membrane protein
Mus musculus
16020
-
2.3.2.2
membrane
intrinsic membrane protein
Rattus norvegicus
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.2.2
22000
-
1 * 51000 + 1 * 22000, Triton X-100 solubilized, SDS-PAGE
Rattus norvegicus
2.3.2.2
22000
-
1 * 46000 + 1 * 22000, papain solubilized, SDS-PAGE
Rattus norvegicus
2.3.2.2
46000
-
1 * 46000 + 1 * 22000, papain solubilized, SDS-PAGE
Rattus norvegicus
2.3.2.2
51000
-
1 * 51000 + 1 * 22000, Triton X-100 solubilized, SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
Mammalia
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
peptide + 5-L-glutamyl amino acid
-
-
r
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
Mus musculus
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
peptide + 5-L-glutamyl amino acid
-
-
r
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
Rattus norvegicus
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
peptide + 5-L-glutamyl amino acid
-
-
r
2.3.2.2
glutathione + an amino acid
Mammalia
involved in 5-L-glutamyl cycle of glutathione metabolism
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Mammalia
ir
2.3.2.2
glutathione + an amino acid
Mus musculus
involved in 5-L-glutamyl cycle of glutathione metabolism
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Mus musculus
ir
2.3.2.2
glutathione + an amino acid
Rattus norvegicus
involved in 5-L-glutamyl cycle of glutathione metabolism
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Rattus norvegicus
ir
2.3.2.2
glutathione + an amino acid
Mammalia
initial step of glutathione degradation
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Mammalia
ir
2.3.2.2
glutathione + an amino acid
Mus musculus
initial step of glutathione degradation
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Mus musculus
ir
2.3.2.2
glutathione + an amino acid
Rattus norvegicus
initial step of glutathione degradation
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Rattus norvegicus
ir
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.2.2
Mammalia
-
-
-
2.3.2.2
Mus musculus
-
-
-
2.3.2.2
Rattus norvegicus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.3.2.2
glycoprotein
-
Mammalia
2.3.2.2
glycoprotein
both subunits are glycoproteins, structure
Rattus norvegicus
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.2.2
solubilized with either detergents or proteinases
Rattus norvegicus
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
2.3.2.2
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
mechanism
Mammalia
2.3.2.2
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
mechanism
Mus musculus
2.3.2.2
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
mechanism
Rattus norvegicus
2.3.2.2
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
structure
Mammalia
2.3.2.2
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
structure
Mus musculus
2.3.2.2
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
structure
Rattus norvegicus
Renatured (Commentary)
EC Number
Renatured (Commentary)
Organism
2.3.2.2
-
Mammalia
2.3.2.2
-
Mus musculus
2.3.2.2
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.2.2
epididymis
-
Mammalia
-
2.3.2.2
epididymis
-
Mus musculus
-
2.3.2.2
epididymis
-
Rattus norvegicus
-
2.3.2.2
intestine
-
Mammalia
-
2.3.2.2
intestine
-
Mus musculus
-
2.3.2.2
intestine
-
Rattus norvegicus
-
2.3.2.2
jejunum
outer space of epithelial membranes
Mammalia
-
2.3.2.2
jejunum
outer space of epithelial membranes
Mus musculus
-
2.3.2.2
jejunum
outer space of epithelial membranes
Rattus norvegicus
-
2.3.2.2
kidney
-
Mammalia
-
2.3.2.2
kidney
-
Mus musculus
-
2.3.2.2
kidney
-
Rattus norvegicus
-
2.3.2.2
liver
-
Mammalia
-
2.3.2.2
liver
-
Mus musculus
-
2.3.2.2
liver
-
Rattus norvegicus
-
2.3.2.2
additional information
distribution
Mammalia
-
2.3.2.2
additional information
distribution
Mus musculus
-
2.3.2.2
additional information
distribution
Rattus norvegicus
-
2.3.2.2
pancreas
-
Mammalia
-
2.3.2.2
pancreas
-
Mus musculus
-
2.3.2.2
pancreas
-
Rattus norvegicus
-
2.3.2.2
seminal plasma
-
Mammalia
-
2.3.2.2
seminal plasma
-
Mus musculus
-
2.3.2.2
seminal plasma
-
Rattus norvegicus
-
2.3.2.2
spleen
-
Mammalia
-
2.3.2.2
spleen
-
Mus musculus
-
2.3.2.2
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.2.2
738
-
purified Triton X-100 solubilized kidney enzyme
Rattus norvegicus
2.3.2.2
810
-
purified papain solubilized kidney enzyme
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor binding site reacts with L- and to a lesser extent D-enantiomer
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor binding site reacts with L- and to a lesser extent D-enantiomer
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor binding site reacts with L- and to a lesser extent D-enantiomer
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
acceptor specificity, overview
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
acceptor specificity, overview
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
acceptor specificity, overview
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
strict L-stereospecificity of 5-Glu-acceptor site
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
strict L-stereospecificity of 5-Glu-acceptor site
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
strict L-stereospecificity of 5-Glu-acceptor site
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor: glutathione disulfide
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor: glutathione disulfide
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor: glutathione disulfide
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
r
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
r
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
r
2.3.2.2
5-L-glutamyl-4-nitroanilide + glycylglycine
-
487931
Mammalia
4-nitroaniline + 5-L-glutamylglycylglycine
-
487931
Mammalia
?
2.3.2.2
5-L-glutamyl-4-nitroanilide + glycylglycine
-
487931
Mus musculus
4-nitroaniline + 5-L-glutamylglycylglycine
-
487931
Mus musculus
?
2.3.2.2
5-L-glutamyl-4-nitroanilide + glycylglycine
-
487931
Rattus norvegicus
4-nitroaniline + 5-L-glutamylglycylglycine
-
487931
Rattus norvegicus
?
2.3.2.2
5-L-glutamyl-4-nitroanilide + L-cystine
-
487931
Rattus norvegicus
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
?
2.3.2.2
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
487931
Mammalia
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
?
2.3.2.2
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
487931
Mus musculus
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
?
2.3.2.2
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
487931
Rattus norvegicus
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
?
2.3.2.2
glutathione + acceptor
acceptor: amino acids
487931
Mammalia
cysteinylglycine + 5-L-glutamyl-acceptor
-
487931
Mammalia
r
2.3.2.2
glutathione + acceptor
acceptor: amino acids
487931
Mus musculus
cysteinylglycine + 5-L-glutamyl-acceptor
-
487931
Mus musculus
r
2.3.2.2
glutathione + acceptor
acceptor: amino acids
487931
Rattus norvegicus
cysteinylglycine + 5-L-glutamyl-acceptor
-
487931
Rattus norvegicus
r
2.3.2.2
glutathione + an amino acid
involved in 5-L-glutamyl cycle of glutathione metabolism
487931
Mammalia
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Mammalia
ir
2.3.2.2
glutathione + an amino acid
involved in 5-L-glutamyl cycle of glutathione metabolism
487931
Mus musculus
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Mus musculus
ir
2.3.2.2
glutathione + an amino acid
involved in 5-L-glutamyl cycle of glutathione metabolism
487931
Rattus norvegicus
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Rattus norvegicus
ir
2.3.2.2
glutathione + an amino acid
initial step of glutathione degradation
487931
Mammalia
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Mammalia
ir
2.3.2.2
glutathione + an amino acid
initial step of glutathione degradation
487931
Mus musculus
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Mus musculus
ir
2.3.2.2
glutathione + an amino acid
initial step of glutathione degradation
487931
Rattus norvegicus
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Rattus norvegicus
ir
2.3.2.2
glutathione + glutathione
autotranspeptidation
487931
Mammalia
L-cysteinylglycine + 5-glutamyl-glutathione
-
487931
Mammalia
r
2.3.2.2
glutathione + glutathione
autotranspeptidation
487931
Mus musculus
L-cysteinylglycine + 5-glutamyl-glutathione
-
487931
Mus musculus
r
2.3.2.2
glutathione + glutathione
autotranspeptidation
487931
Rattus norvegicus
L-cysteinylglycine + 5-glutamyl-glutathione
-
487931
Rattus norvegicus
r
2.3.2.2
glutathione + H2O
hydrolase reaction, concurrent to (auto-)transpeptidation
487931
Mammalia
L-cysteinylglycine + L-glutamate
-
487931
Mammalia
ir
2.3.2.2
glutathione + H2O
hydrolase reaction, concurrent to (auto-)transpeptidation
487931
Mus musculus
L-cysteinylglycine + L-glutamate
-
487931
Mus musculus
ir
2.3.2.2
glutathione + H2O
hydrolase reaction, concurrent to (auto-)transpeptidation
487931
Rattus norvegicus
L-cysteinylglycine + L-glutamate
-
487931
Rattus norvegicus
ir
2.3.2.2
L-Glu-4-nitroanilide + glutathione
-
487931
Rattus norvegicus
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.2.2
dimer
1 * 51000 + 1 * 22000, Triton X-100 solubilized, SDS-PAGE
Rattus norvegicus
2.3.2.2
dimer
1 * 46000 + 1 * 22000, papain solubilized, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.2.2
additional information
-
12 isozymes with pI-values ranging from 5 to 8
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.2.2
additional information
-
additional information
Ki values for diverse amino acids, overview
Mammalia
2.3.2.2
additional information
-
additional information
Ki values for diverse amino acids, overview
Mus musculus
2.3.2.2
additional information
-
additional information
Ki values for diverse amino acids, overview
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.2.2
Free bile acids
activation
Mammalia
2.3.2.2
Free bile acids
activation
Mus musculus
2.3.2.2
Free bile acids
activation
Rattus norvegicus
2.3.2.2
Free bile acids
and their glycine and taurine conjugates
Mammalia
2.3.2.2
Free bile acids
and their glycine and taurine conjugates
Mus musculus
2.3.2.2
Free bile acids
and their glycine and taurine conjugates
Rattus norvegicus
2.3.2.2
hippurate
stimulates hydrolase reaction, inhibits transpeptidation
Mammalia
2.3.2.2
hippurate
stimulates hydrolase reaction, inhibits transpeptidation
Mus musculus
2.3.2.2
hippurate
stimulates hydrolase reaction, inhibits transpeptidation
Rattus norvegicus
2.3.2.2
Maleate
-
Mammalia
2.3.2.2
Maleate
-
Mus musculus
2.3.2.2
Maleate
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.2.2
5-glutamylhydrazones
-
Mammalia
2.3.2.2
5-glutamylhydrazones
-
Mus musculus
2.3.2.2
5-glutamylhydrazones
-
Rattus norvegicus
2.3.2.2
5-glutamylphenylhydrazides
-
Mammalia
2.3.2.2
5-glutamylphenylhydrazides
-
Mus musculus
2.3.2.2
5-glutamylphenylhydrazides
-
Rattus norvegicus
2.3.2.2
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective
Mammalia
2.3.2.2
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective
Mus musculus
2.3.2.2
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective
Rattus norvegicus
2.3.2.2
6-diazo-5-oxo-L-norleucine
-
Mammalia
2.3.2.2
6-diazo-5-oxo-L-norleucine
-
Mus musculus
2.3.2.2
6-diazo-5-oxo-L-norleucine
-
Rattus norvegicus
2.3.2.2
Acetazolamide Maleate
transpeptidase, not hydrolase reaction
Mammalia
2.3.2.2
Acetazolamide Maleate
transpeptidase, not hydrolase reaction
Mus musculus
2.3.2.2
Acetazolamide Maleate
transpeptidase, not hydrolase reaction
Rattus norvegicus
2.3.2.2
Free bile acids and their glycine and taurine conjugates
-
Mammalia
2.3.2.2
Free bile acids and their glycine and taurine conjugates
-
Mus musculus
2.3.2.2
Free bile acids and their glycine and taurine conjugates
-
Rattus norvegicus
2.3.2.2
hippurate
transpeptidase, not hydrolase reaction
Mammalia
2.3.2.2
hippurate
transpeptidase, not hydrolase reaction
Mus musculus
2.3.2.2
hippurate
transpeptidase, not hydrolase reaction
Rattus norvegicus
2.3.2.2
L- or D-Serine/borate
-
Mammalia
2.3.2.2
L- or D-Serine/borate
-
Mus musculus
2.3.2.2
L- or D-Serine/borate
-
Rattus norvegicus
2.3.2.2
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
i.e. AT-125
Mammalia
2.3.2.2
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
i.e. AT-125
Mus musculus
2.3.2.2
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
i.e. AT-125
Rattus norvegicus
2.3.2.2
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
strong, irreversible
Mammalia
2.3.2.2
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
strong, irreversible
Mus musculus
2.3.2.2
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
strong, irreversible
Rattus norvegicus
2.3.2.2
L-azaserine
irreversible inactivation
Mammalia
2.3.2.2
L-azaserine
irreversible inactivation
Mus musculus
2.3.2.2
L-azaserine
irreversible inactivation
Rattus norvegicus
2.3.2.2
additional information
-
Mammalia
2.3.2.2
additional information
-
Mus musculus
2.3.2.2
additional information
-
Rattus norvegicus
2.3.2.2
PMSF
-
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.2.2
additional information
-
additional information
Ki values for diverse amino acids, overview
Mammalia
2.3.2.2
additional information
-
additional information
Ki values for diverse amino acids, overview
Mus musculus
2.3.2.2
additional information
-
additional information
Ki values for diverse amino acids, overview
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.2.2
additional information
-
additional information
-
Mammalia
2.3.2.2
additional information
-
additional information
-
Mus musculus
2.3.2.2
additional information
-
additional information
-
Rattus norvegicus
2.3.2.2
0.005
0.0068
L-Glu-4-nitroanilide
hydrolase reaction
Rattus norvegicus
2.3.2.2
0.029
0.035
L-cystine
hydrolase reaction
Rattus norvegicus
2.3.2.2
0.029
0.035
L-cystine
D-Glu-4-nitroanilide
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.2.2
brush border
jejunum, microvilli
Mammalia
5903
-
2.3.2.2
brush border
jejunum, microvilli
Mus musculus
5903
-
2.3.2.2
brush border
jejunum, microvilli
Rattus norvegicus
5903
-
2.3.2.2
extracellular
-
Mammalia
-
-
2.3.2.2
extracellular
-
Mus musculus
-
-
2.3.2.2
extracellular
-
Rattus norvegicus
-
-
2.3.2.2
membrane
intrinsic membrane protein
Mammalia
16020
-
2.3.2.2
membrane
intrinsic membrane protein
Mus musculus
16020
-
2.3.2.2
membrane
intrinsic membrane protein
Rattus norvegicus
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.2.2
22000
-
1 * 51000 + 1 * 22000, Triton X-100 solubilized, SDS-PAGE
Rattus norvegicus
2.3.2.2
22000
-
1 * 46000 + 1 * 22000, papain solubilized, SDS-PAGE
Rattus norvegicus
2.3.2.2
46000
-
1 * 46000 + 1 * 22000, papain solubilized, SDS-PAGE
Rattus norvegicus
2.3.2.2
51000
-
1 * 51000 + 1 * 22000, Triton X-100 solubilized, SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
Mammalia
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
peptide + 5-L-glutamyl amino acid
-
-
r
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
Mus musculus
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
peptide + 5-L-glutamyl amino acid
-
-
r
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
Rattus norvegicus
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
peptide + 5-L-glutamyl amino acid
-
-
r
2.3.2.2
glutathione + an amino acid
Mammalia
involved in 5-L-glutamyl cycle of glutathione metabolism
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Mammalia
ir
2.3.2.2
glutathione + an amino acid
Mus musculus
involved in 5-L-glutamyl cycle of glutathione metabolism
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Mus musculus
ir
2.3.2.2
glutathione + an amino acid
Rattus norvegicus
involved in 5-L-glutamyl cycle of glutathione metabolism
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Rattus norvegicus
ir
2.3.2.2
glutathione + an amino acid
Mammalia
initial step of glutathione degradation
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Mammalia
ir
2.3.2.2
glutathione + an amino acid
Mus musculus
initial step of glutathione degradation
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Mus musculus
ir
2.3.2.2
glutathione + an amino acid
Rattus norvegicus
initial step of glutathione degradation
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Rattus norvegicus
ir
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.3.2.2
glycoprotein
-
Mammalia
2.3.2.2
glycoprotein
both subunits are glycoproteins, structure
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.2.2
solubilized with either detergents or proteinases
Rattus norvegicus
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.2.2
-
Mammalia
2.3.2.2
-
Mus musculus
2.3.2.2
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.2.2
epididymis
-
Mammalia
-
2.3.2.2
epididymis
-
Mus musculus
-
2.3.2.2
epididymis
-
Rattus norvegicus
-
2.3.2.2
intestine
-
Mammalia
-
2.3.2.2
intestine
-
Mus musculus
-
2.3.2.2
intestine
-
Rattus norvegicus
-
2.3.2.2
jejunum
outer space of epithelial membranes
Mammalia
-
2.3.2.2
jejunum
outer space of epithelial membranes
Mus musculus
-
2.3.2.2
jejunum
outer space of epithelial membranes
Rattus norvegicus
-
2.3.2.2
kidney
-
Mammalia
-
2.3.2.2
kidney
-
Mus musculus
-
2.3.2.2
kidney
-
Rattus norvegicus
-
2.3.2.2
liver
-
Mammalia
-
2.3.2.2
liver
-
Mus musculus
-
2.3.2.2
liver
-
Rattus norvegicus
-
2.3.2.2
additional information
distribution
Mammalia
-
2.3.2.2
additional information
distribution
Mus musculus
-
2.3.2.2
additional information
distribution
Rattus norvegicus
-
2.3.2.2
pancreas
-
Mammalia
-
2.3.2.2
pancreas
-
Mus musculus
-
2.3.2.2
pancreas
-
Rattus norvegicus
-
2.3.2.2
seminal plasma
-
Mammalia
-
2.3.2.2
seminal plasma
-
Mus musculus
-
2.3.2.2
seminal plasma
-
Rattus norvegicus
-
2.3.2.2
spleen
-
Mammalia
-
2.3.2.2
spleen
-
Mus musculus
-
2.3.2.2
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.2.2
738
-
purified Triton X-100 solubilized kidney enzyme
Rattus norvegicus
2.3.2.2
810
-
purified papain solubilized kidney enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor binding site reacts with L- and to a lesser extent D-enantiomer
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor binding site reacts with L- and to a lesser extent D-enantiomer
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor binding site reacts with L- and to a lesser extent D-enantiomer
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
acceptor specificity, overview
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
acceptor specificity, overview
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
acceptor specificity, overview
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
strict L-stereospecificity of 5-Glu-acceptor site
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
strict L-stereospecificity of 5-Glu-acceptor site
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
strict L-stereospecificity of 5-Glu-acceptor site
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor: glutathione disulfide
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor: glutathione disulfide
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
donor: glutathione disulfide
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
?
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
487931
Mammalia
peptide + 5-L-glutamyl amino acid
-
-
-
r
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
487931
Mus musculus
peptide + 5-L-glutamyl amino acid
-
-
-
r
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
487931
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
r
2.3.2.2
5-L-glutamyl-4-nitroanilide + glycylglycine
-
487931
Mammalia
4-nitroaniline + 5-L-glutamylglycylglycine
-
487931
Mammalia
?
2.3.2.2
5-L-glutamyl-4-nitroanilide + glycylglycine
-
487931
Mus musculus
4-nitroaniline + 5-L-glutamylglycylglycine
-
487931
Mus musculus
?
2.3.2.2
5-L-glutamyl-4-nitroanilide + glycylglycine
-
487931
Rattus norvegicus
4-nitroaniline + 5-L-glutamylglycylglycine
-
487931
Rattus norvegicus
?
2.3.2.2
5-L-glutamyl-4-nitroanilide + L-cystine
-
487931
Rattus norvegicus
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
?
2.3.2.2
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
487931
Mammalia
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
?
2.3.2.2
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
487931
Mus musculus
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
?
2.3.2.2
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
487931
Rattus norvegicus
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
?
2.3.2.2
glutathione + acceptor
acceptor: amino acids
487931
Mammalia
cysteinylglycine + 5-L-glutamyl-acceptor
-
487931
Mammalia
r
2.3.2.2
glutathione + acceptor
acceptor: amino acids
487931
Mus musculus
cysteinylglycine + 5-L-glutamyl-acceptor
-
487931
Mus musculus
r
2.3.2.2
glutathione + acceptor
acceptor: amino acids
487931
Rattus norvegicus
cysteinylglycine + 5-L-glutamyl-acceptor
-
487931
Rattus norvegicus
r
2.3.2.2
glutathione + an amino acid
involved in 5-L-glutamyl cycle of glutathione metabolism
487931
Mammalia
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Mammalia
ir
2.3.2.2
glutathione + an amino acid
involved in 5-L-glutamyl cycle of glutathione metabolism
487931
Mus musculus
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Mus musculus
ir
2.3.2.2
glutathione + an amino acid
involved in 5-L-glutamyl cycle of glutathione metabolism
487931
Rattus norvegicus
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Rattus norvegicus
ir
2.3.2.2
glutathione + an amino acid
initial step of glutathione degradation
487931
Mammalia
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Mammalia
ir
2.3.2.2
glutathione + an amino acid
initial step of glutathione degradation
487931
Mus musculus
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Mus musculus
ir
2.3.2.2
glutathione + an amino acid
initial step of glutathione degradation
487931
Rattus norvegicus
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
487931
Rattus norvegicus
ir
2.3.2.2
glutathione + glutathione
autotranspeptidation
487931
Mammalia
L-cysteinylglycine + 5-glutamyl-glutathione
-
487931
Mammalia
r
2.3.2.2
glutathione + glutathione
autotranspeptidation
487931
Mus musculus
L-cysteinylglycine + 5-glutamyl-glutathione
-
487931
Mus musculus
r
2.3.2.2
glutathione + glutathione
autotranspeptidation
487931
Rattus norvegicus
L-cysteinylglycine + 5-glutamyl-glutathione
-
487931
Rattus norvegicus
r
2.3.2.2
glutathione + H2O
hydrolase reaction, concurrent to (auto-)transpeptidation
487931
Mammalia
L-cysteinylglycine + L-glutamate
-
487931
Mammalia
ir
2.3.2.2
glutathione + H2O
hydrolase reaction, concurrent to (auto-)transpeptidation
487931
Mus musculus
L-cysteinylglycine + L-glutamate
-
487931
Mus musculus
ir
2.3.2.2
glutathione + H2O
hydrolase reaction, concurrent to (auto-)transpeptidation
487931
Rattus norvegicus
L-cysteinylglycine + L-glutamate
-
487931
Rattus norvegicus
ir
2.3.2.2
L-Glu-4-nitroanilide + glutathione
-
487931
Rattus norvegicus
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.2.2
dimer
1 * 51000 + 1 * 22000, Triton X-100 solubilized, SDS-PAGE
Rattus norvegicus
2.3.2.2
dimer
1 * 46000 + 1 * 22000, papain solubilized, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.2.2
additional information
-
12 isozymes with pI-values ranging from 5 to 8
Rattus norvegicus