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Literature summary extracted from

  • Zelazo, P.; Orlowski, M.
    gamma-Glutamyl transpeptidase of sheep-kidney cortex. Isolation, catalytic properties and dissociation into two polypeptide chains (1976), Eur. J. Biochem., 61, 147-155.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 brush border kidney Ovis aries 5903
-
2.3.2.2 membrane
-
 Ovis aries 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.2 Ca2+ activation, transpeptidase Ovis aries
2.3.2.2 Ca2+ not hydrolase Ovis aries
2.3.2.2 K+ activation, transpeptidase Ovis aries
2.3.2.2 K+ not hydrolase Ovis aries
2.3.2.2 Li+ activation Ovis aries
2.3.2.2 Mg2+ activation, transpeptidase Ovis aries
2.3.2.2 Mg2+ not hydrolase Ovis aries
2.3.2.2 Na+ activation, transpeptidase Ovis aries
2.3.2.2 Na+ not hydrolase Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 27000
-
 1 * 65000 + 1 * 27000, SDS-PAGE, 8 M urea-PAGE Ovis aries
2.3.2.2 65000
-
 1 * 65000 + 1 * 27000, SDS-PAGE, 8 M urea-PAGE Ovis aries
2.3.2.2 90000
-
 PAGE in 8 M urea, after cross-linkage of subunits with dimethylsuberimidate Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 kidney cortex Ovis aries
-
2.3.2.2 kidney brush border of convoluted tubules Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 510
-
 purified enzyme, without acceptor Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor specificity, overview Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide autotranspeptidation, L- or, to a lesser extent, D-enantiomer Ovis aries 5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycine 19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors Ovis aries 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Ovis aries 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-alanine L-Ala or D-Ala Ovis aries 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-arginine
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-asparagine
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-aspartate
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-aspartate
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-histidine
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-histidine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-isoleucine
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-leucine
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-leucine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-lysine
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-methionine 44% the rate of the reaction with glycylglycine, L-Met or D-Met Ovis aries 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-phenylalanine
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-valine
-
 Ovis aries 4-nitroaniline + 5-L-glutamyl-L-valine
-
 ?
2.3.2.2 glutathione + acceptor
-
 Ovis aries cysteinylglycine + 5-L-glutamyl-acceptor
-
 r
2.3.2.2 glutathione + glutathione autotranspeptidation Ovis aries L-cysteinylglycine + 5-glutamyl-glutathione
-
 r
2.3.2.2 glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Ovis aries L-cysteinylglycine + L-glutamate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 65000 + 1 * 27000, SDS-PAGE, 8 M urea-PAGE Ovis aries

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 8.8
-
 transpeptidation Ovis aries
2.3.2.2 9.5
-
 hydrolase reaction Ovis aries