Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Furukawa, M.; Higashi, T.; Tateishi, N.; Ochi, K.; Sakamoto, Y.
    Purification and properties of bovine liver gamma-glutamyltransferase (1983), J. Biochem., 93, 839-846.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 glutathione complete inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.8
-
 L-Glu-4-nitroanilide in presence of glycylglycine Bos taurus
2.3.2.2 1.25
-
 L-Glu-4-nitroanilide in absence of glycylglycine Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 membrane
-
 Bos taurus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 additional information
-
 amino acid composition Bos taurus
2.3.2.2 27000
-
 1 * 68000 + 1 * 27000, SDS-PAGE Bos taurus
2.3.2.2 68000
-
 1 * 68000 + 1 * 27000, SDS-PAGE Bos taurus
2.3.2.2 110000
-
 PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein sialoglycoprotein Bos taurus
2.3.2.2 glycoprotein rich in carbohydrates Bos taurus
2.3.2.2 glycoprotein contains neutral and amino sugars and sialic acid Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 956
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Bos taurus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Bos taurus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Bos taurus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-alanine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-arginine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-cysteine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-cysteine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-lysine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-methionine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-phenylalanine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 68000 + 1 * 27000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 25
-
 assay at Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.2 0.3
-
 glutathione
-
 Bos taurus