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Literature summary extracted from

  • Takahashi, S.; Steinman, H.M.; Ball, D.
    Purification and characterization of gamma-glutamyltransferase from rat pancreas (1982), Biochim. Biophys. Acta, 707, 66-73.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 5-Iodoacetamidofluorescein
-
 Rattus norvegicus
2.3.2.2 6-diazo-5-oxo-L-norleucine irreversible inactivation of pancreatic enzyme, S-methylglutathione prevents at 10 mM, maleate increases inhibition at 0.25-1 M Rattus norvegicus
2.3.2.2 Antiserum to rat kidney glutamyltransferase
-
 Rattus norvegicus
2.3.2.2 L- or D-Serine/borate reversible Rattus norvegicus
2.3.2.2 L-azaserine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.9
-
 L-Glu-4-nitroanilide
-
 Rattus norvegicus
2.3.2.2 20
-
 glycylglycine
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 additional information
-
 amino acid composition Rattus norvegicus
2.3.2.2 25000
-
 1 * 43000 + 1 * 25000, SDS-PAGE Rattus norvegicus
2.3.2.2 43000
-
 1 * 43000 + 1 * 25000, SDS-PAGE Rattus norvegicus
2.3.2.2 68000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Rattus norvegicus
-
 Wistar
-
2.3.2.2 Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 pancreas
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 630
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Rattus norvegicus Wistar peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
-
 Rattus norvegicus 5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
-
 Rattus norvegicus Wistar 5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Rattus norvegicus Wistar 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Rattus norvegicus Wistar 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 43000 + 1 * 25000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 8
-
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.2 1.2
-
 6-diazo-5-oxo-L-norleucine
-
 Rattus norvegicus