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Literature summary extracted from

  • Kimm, S.W.; Kim, E.G.; Park, S.C.
    Partial purification and characterization of gamma-glutamyl transpeptidase from rat kidney (1986), Korean J. Biochem., 18, 135-143.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 Ca2+ no inhibition Rattus norvegicus
2.3.2.2 glycine strong Rattus norvegicus
2.3.2.2 additional information no inhibition by phorbol ester, polyamines, cAMP Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.21
-
 L-Glu-4-nitroanilide with 8 mM L-Gly-Gly, kidney enzyme Rattus norvegicus
2.3.2.2 0.32
-
 L-Glu-4-nitroanilide with 16 mM L-Gly-Gly , kidney enzyme Rattus norvegicus
2.3.2.2 4
-
 glycylglycine with 0.8 mM L-Glu-4-nitroanilide, kidney enzyme Rattus norvegicus
2.3.2.2 4.35
-
 glycylglycine with 1.6 mM L-Glu-4-nitroanilide, kidney enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 brush border kidney Rattus norvegicus 5903
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 22000
-
 1 * 50000 + 1 * 22000, kidney, SDS-PAGE Rattus norvegicus
2.3.2.2 50000
-
 1 * 50000 + 1 * 22000, kidney, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 540
-
 kidney Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamine L-methionine and L-glutamine are best acceptors Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-methionine L-methionine and glutamine are best acceptors Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 50000 + 1 * 22000, kidney, SDS-PAGE Rattus norvegicus