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Literature summary extracted from

  • Kang, H.; Cho, Y.D.
    Purification and properties of transglutaminase from soybean (Glycine max) leaves (1996), Biochem. Biophys. Res. Commun., 223, 288-292.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.13 dithiothreitol 10 mM, approx. 1.8fold activation Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 GTP
-
 Glycine max
2.3.2.13 N-ethylmaleimide 0.1 mM, 60% inhibition Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 0.042
-
 spermidine
-
 Glycine max
2.3.2.13 0.069
-
 spermine
-
 Glycine max
2.3.2.13 0.11
-
 putrescine
-
 Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 additional information Ca2+ is not required for activity Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 80000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 ammonium sulfate, DEAE-Sepharose, Blue-Sepharose CL-6B, omega-aminohexyl agarose, alpha-casein agarose Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 leaf
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 0.16
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 protein-bound gamma-glutamine + alkylamine
-
 Glycine max protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 putrescine + N,N'-dimethylcasein spermine and spermidine can replace putrescine Glycine max ?
-
 ?
2.3.2.13 spermidine + N,N'-dimethylcasein
-
 Glycine max ?
-
 ?
2.3.2.13 spermine + N,N'-dimethylcasein
-
 Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 monomer 1 * 80000, SDS-PAGE Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 37
-
-
 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 7.6
-
-
 Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.13 0.9
-
 GTP
-
 Glycine max