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Literature summary extracted from

  • Tokunaga, F.; Yamada, M.; Miyata, T.; Ding, Y.L.; Hiranaga-Kawabata, M.; Muta, T.; Iwanaga, S.
    Limulus hemocyte transglutaminase. Its purification and characterization, and identification of the intracellular substrates (1993), J. Biol. Chem., 268, 252-261.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.13 dithiothreitol weak activation Tachypleus tridentatus

General Stability

EC Number General Stability Organism
2.3.2.13 loss of activity after freezing Tachypleus tridentatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 Cu2+ 1 mM, complete inhibition Tachypleus tridentatus
2.3.2.13 EDTA 5 mM; 5 mM, complete inhibition Tachypleus tridentatus
2.3.2.13 hydroxylamine 100 mM, complete inhibition Tachypleus tridentatus
2.3.2.13 KCl above 500 mM Tachypleus tridentatus
2.3.2.13 monoiodoacetate 1 mM, 94% inhibition Tachypleus tridentatus
2.3.2.13 additional information not inhibited by diisopropylfluorophosphate Tachypleus tridentatus
2.3.2.13 N-ethylmaleimide 1 mM, 98% inhibition Tachypleus tridentatus
2.3.2.13 NaCl above 500 mM Tachypleus tridentatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.13 soluble
-
 Tachypleus tridentatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ requirement Tachypleus tridentatus
2.3.2.13 Ca2+ 8 mM, approx. 10fold activation Tachypleus tridentatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 86000
-
 1 * 86000, SDS-PAGE Tachypleus tridentatus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Tachypleus tridentatus
-
 japanese horseshoe crab
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 CM-Sepharose CL-6B, ammonium sulfate, DEAE-cellulose, Sephacryl S-300, DEAE-cosmogel, Zn-chelating Sepharose Tachypleus tridentatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 hemocyte
-
 Tachypleus tridentatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 additional information
-
 938 amine incorporation units/min Tachypleus tridentatus

Storage Stability

EC Number Storage Stability Organism
2.3.2.13 4°C, at least 3 months Tachypleus tridentatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 protein-bound gamma-glutamine + alkylamine identification of natural protein substrates Tachypleus tridentatus protein N5-alkylglutamine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 monomer 1 * 86000, SDS-PAGE Tachypleus tridentatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 37
-
 assay at Tachypleus tridentatus