Literature summary extracted from

Traut, R.R.; Monro, R.E.
The puromycin reaction and its relation to protein synthesis (1964), J. Mol. Biol., 10, 63-72.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.3.2.12	K+	reaction is completely dependent upon potassium ions	Escherichia coli
2.3.2.12	NH4+	reaction is completely dependent upon ammonium ions	Escherichia coli

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.3.2.12	chloramphenicol	50% inhibition	Escherichia coli
2.3.2.12	chlortetracycline	59% inhibition	Escherichia coli
2.3.2.12	EDTA	-	Escherichia coli

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.3.2.12	ribosome	50S subunit	Escherichia coli	5840	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.3.2.12	Mg2+	-	Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.3.2.12	peptidyl-tRNA1 + alpha-aminoacyl-tRNA2	Escherichia coli	-	tRNA1 + peptidyl-amino-tRNA2	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.2.12	Escherichia coli	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.2.12	peptidyl-tRNA1 + alpha-aminoacyl-tRNA2	-	Escherichia coli	tRNA1 + peptidyl-amino-tRNA2	-	?
2.3.2.12	polyphenylalanyl-tRNA + puromycin	-	Escherichia coli	tRNA + polyphenylalanyl-puromycin	-	?

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.3.2.12	30	-	enzyme assay	Escherichia coli

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Release 2023.1 (January 2023)