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Literature summary extracted from

  • Hegde, S.S.; Blanchard, J.S.
    Kinetic and mechanistic characterization of recombinant Lactobacillus viridescens FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase) (2003), J. Biol. Chem., 278, 22861-22867.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.10 expression of wild-type and mutants as His-tagged proteins in Escherichia coli, in vitro transcription of tRNAs Weissella viridescens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.10 D109N site-directed mutagenesis, loss of 99% activity compared to the wild-type Weissella viridescens
2.3.2.10 E205Q site-directed mutagenesis, unaltered activity compared to the wild-type Weissella viridescens
2.3.2.10 E215Q site-directed mutagenesis, loss of 15% activity compared to the wild-type Weissella viridescens
2.3.2.10 E316Q site-directed mutagenesis, loss of 60% activity compared to the wild-type Weissella viridescens
2.3.2.10 E320Q site-directed mutagenesis, loss of 96% activity compared to the wild-type Weissella viridescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.10 Ser-tRNA versus UDP-acetylmuramoylpentapeptide Weissella viridescens
2.3.2.10 UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine product inhibition Weissella viridescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.10 0.015
-
 L-alanyl-tRNA(Ala) Ala-tRNAAla from E. coli, recombinant wild-type Weissella viridescens
2.3.2.10 0.042
-
 UDP-acetylmuramoylpentapeptide recombinant wild-type Weissella viridescens
2.3.2.10 0.054
-
 L-alanyl-tRNA(Ala) Ala-tRNAAla from E. coli, recombinant mutant E320Q Weissella viridescens
2.3.2.10 0.061
-
 L-alanyl-tRNA(Ala) Ala-tRNAAla from E. coli, recombinant mutant D109N Weissella viridescens
2.3.2.10 0.067
-
 UDP-acetylmuramoylpentapeptide recombinant mutant E320Q Weissella viridescens
2.3.2.10 0.165
-
 UDP-acetylmuramoylpentapeptide recombinant mutant D109N Weissella viridescens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.10 Weissella viridescens
-
 femX gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.10 recombinant His-tagged wild-type and mutants from E. coli Weissella viridescens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.2.10 L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine transfer of L-Ala to epsilon-amino group of L-lysine of UDP-UDP-acetylmuramoylpentapeptide in an ordered sequential mechanism Weissella viridescens
a
2.3.2.10 L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine catalysis via a ternary complex Weissella viridescens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.10 L-alanyl-tRNA(Ala) + UDP-N-acetylmuramoyl-pentapeptide containing L-lysine
-
 Weissella viridescens ?
-
 r
2.3.2.10 L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
 Weissella viridescens tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
2.3.2.10 L-alanyl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
 Weissella viridescens tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
2.3.2.10 L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
 Weissella viridescens tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
-
 ?
2.3.2.10 additional information also utilizes UDP-N-acetylmuramoylpentapeptide containing L-ornithine as substrate Weissella viridescens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.10 25
-
 assay at Weissella viridescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.10 0.183
-
 L-alanyl-tRNAAla recombinant mutant D109N Weissella viridescens
2.3.2.10 0.917
-
 L-alanyl-tRNAAla recombinant mutant E320Q Weissella viridescens
2.3.2.10 11
-
 L-alanyl-tRNAAla recombinant wild-type Weissella viridescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.10 additional information
-
 broad maximum Weissella viridescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.10 additional information
-
 pK values of 5.5 and 9.3 Weissella viridescens
2.3.2.10 5.5 9.1
-
 Weissella viridescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.10 additional information
-
 additional information
-
 Weissella viridescens
2.3.2.10 0.048
-
 UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine product inhibition Weissella viridescens
2.3.2.10 0.248
-
 Ser-tRNA versus UDP-acetylmuramoylpentapeptide Weissella viridescens