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Literature summary extracted from

  • Strack, D.; Gross, W.
    Properties and activity changes of chlorogenic acid:glucaric acid caffeoyltransferase from tomato (Lycopersicon esculentum) (1990), Plant Physiol., 92, 41-47.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.3.1.98 freezing and thawing causes 20-30% loss of activity Solanum lycopersicum
2.3.1.98 purification has to be carried out at 4°C Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.98 additional information p-chloromercuribenzoate is no inhibitor Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.98 0.4
-
 glucaric acid
-
 Solanum lycopersicum
2.3.1.98 1.7
-
 galactaric acid
-
 Solanum lycopersicum
2.3.1.98 20
-
 chlorogenic acid
-
 Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.98 Ca2+ 150% of activity compared to the activity without addition of divalent ions Solanum lycopersicum
2.3.1.98 Mg2+ 125% of activity compared to the activity without addition of divalent ions Solanum lycopersicum
2.3.1.98 additional information EDTA has no effect Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.98 40000
-
 SDS-PAGE, gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.98 5-O-caffeoylquinic acid + galactaric acid Solanum lycopersicum
-
 quinate + caffeoylgalactaric acid
-
 ?
2.3.1.98 5-O-caffeoylquinic acid + glucaric acid Solanum lycopersicum
-
 quinate + caffeoylglucaric acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.98 Solanum lycopersicum
-
 tomato
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.98 2400fold Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.98 cotyledon
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.98 additional information
-
-
 Solanum lycopersicum

Storage Stability

EC Number Storage Stability Organism
2.3.1.98 -20°C, several months, no apparent loss of activity with crude extract or purified enzyme Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.98 5-O-caffeoylquinic acid + galactaric acid
-
 Solanum lycopersicum quinate + caffeoylgalactaric acid
-
 ?
2.3.1.98 5-O-caffeoylquinic acid + glucaric acid
-
 Solanum lycopersicum quinate + caffeoylglucaric acid
-
 ?
2.3.1.98 5-O-caffeoylquinic acid + glucaric acid i.e. chlorogenic acid Solanum lycopersicum quinate + caffeoylglucaric acid
-
 ?
2.3.1.98 chlorogenic acid + glucarate
-
 Solanum lycopersicum quinate + 2-O-caffeoylglucarate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.98 38
-
-
 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.98 5.7
-
-
 Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.98 4.2 6.2 50% of maximal activity at pH 4.2 and pH 6.2 Solanum lycopersicum