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Literature summary extracted from

  • Raju, R.V.S.; Datla, R.S.S.; Warrington, R.C.; Sharma, R.K.
    Effects of L-histidine and its structural analogs on human N-myristoyltransferase activity and importance of EEVEH amino acid sequence for enzyme activity (1998), Biochemistry, 37, 14928-14936.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.97 L-histidine activation in a concentration dependent manner Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.97 expression of wild-type and mutants from Escherichia coli DH5alpha Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.97 E289G site-directed mutagenesis, 37.8% remaining activity Homo sapiens
2.3.1.97 E289G/E290G site-directed mutagenesis, no remaining activity Homo sapiens
2.3.1.97 E289G/E290G/E292G site-directed mutagenesis, no remaining activity Homo sapiens
2.3.1.97 E289G/E292G site-directed mutagenesis, 7% remaining activity Homo sapiens
2.3.1.97 E290G site-directed mutagenesis, no remaining activity Homo sapiens
2.3.1.97 E292G site-directed mutagenesis, 77.6% remaining activity Homo sapiens
2.3.1.97 E292H site-directed mutagenesis, 76% remaining activity Homo sapiens
2.3.1.97 H293G site-directed mutagenesis, no remaining activity Homo sapiens
2.3.1.97 H293N site-directed mutagenesis, no remaining activity Homo sapiens
2.3.1.97 V291G site-directed mutagenesis, no remaining activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 histamine noncompetitive Homo sapiens
2.3.1.97 L-histidinol noncompetitive, reversible by L-histidine Homo sapiens
2.3.1.97 additional information inhibition mechanism Homo sapiens
2.3.1.97 additional information
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.97 0.195
-
 L-histidine
-
 Homo sapiens
2.3.1.97 0.2
-
 cAMP-dependent protein kinase-derived peptide
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Homo sapiens
-
-
-
2.3.1.97 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.97 recombinant His-tagged wild-type from Escherichia coli Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.97 tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] Glu290, Val-291 and His293 within conserved region EEVEH, are essential for catalysis Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoyl-CoA + glycylpeptide
-
 Homo sapiens N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide
-
 Rattus norvegicus N-myristoylglycylpeptide + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 NMT
-
 Homo sapiens
2.3.1.97 NMT
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.97 30
-
 assay at Homo sapiens